Lipopolysaccharide

DATA No : CLS0423 INFORMANT : Yoshio Kumazawa

NAME : 2'N-3''(dodecanoyloxy)tetradecanoyl, 3'-O-3''(tetradecanoyloxy)tetradecanoyl b-(1

6) D-glucosaminyl 2N-3''hydroxytetradecanoyl,3-O-3''hydroxytetradecanoyl D-glucosamine 1,4'-bisphosphate

COMMON NAME	:
SYMBOL	:	bisphosphorylated disaccharide b-D-GlcpN-4-P-(16)-a-D-GlcpN-1-P
FORMULA	:	C₉₄H₁₇₆O₂₅N₂P₂ MOL.WT (average) : 1796.349

BIOLOGICAL ACTIVITY

Antibody obtained after immunization with the J-5 mutant of Escherichia coli O111 was suited to induce cross-reactive lipid A(Ref. 0425)

PHYSICAL AND CHEMICAL PROPERTIES

SPECTRAL DATA

UV SPECTRA	:

IR SPECTRA	:

NMR SPECTRA	:	¹³C-NMR spectrum of the hydrophilic backbone from lipid A [Spectrum 0023] ¹H,¹H-COSY-NMR spectrum of the hydrophilic backbone from lipid A [Spectrum 0024] ¹H,¹³C-COSY-NMR spectrum of the hydrophilic backbone from lipid A [Spectrum 0025]

MASS SPECTRA	:	Laser-desorption mass spectrum of dephosphorylated lipid A [Spectrum 0026]

OTHER SPECTRA	:

CHROMATOGRAM DATA

SOURCE

Escherichia coli J-5(Ref. 0423)

CHEMICAL SYNTHESIS

METABOLISM

GENETIC INFORMATION

NOTE

¹H-NMR data
[Table 0015]
¹³C-NMR data
[Table 0016]

REFERENCES

[0423]

AUTHOR	:	Holst, O., Muller-Loennies, S., Lindner, B., and Brade, H.
TITLE	:	Chemical structure of the lipid A of Escherichia coli J-5 PubMed ID:8319680
JOURNAL	:	Eur J Biochem.
VOL	:	214 PAGE : 695-701 (1993)

[0425]

AUTHOR	:	Ziegler, E. J., Douglas, H., and Braude, A. I.
TITLE	:	Human antiserum for prevention of the local shwartzman reaction and death from bacterial lipopolysaccharides PubMed ID:4584346
JOURNAL	:	J Clin Invest.
VOL	:	52 PAGE : 3236-3238 (1973)