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Lipoprotein


DATA No : TLP1301 INFORMANT : Kazuo Kondo

NAME : very low density lipoprotein

COMMON NAME: pre b lipoprotein
SYMBOL: VLDL
FORMULA: MOL.WT (average) :
BIOLOGICAL ACTIVITY
Biological activities
VLDL is the major vehicle in the plasma which carries triacylglycerol synthesized in the liver to peripheral tissues for utilization. .
(Ref. 1307)
PHYSICAL AND CHEMICAL PROPERTIES
MELTING POINT:Definition
VLDL is defined as the lipoprotein fraction with the density range of 0.93 to1.006 g/ml.
(Ref. 1301)

BOILING POINT:

REFRACTIVE INDEX:

OPTICAL ROTATION:

DENSITY:0.93 to1.006 g/ml
(Ref. 1301)

SOLUBILITY:Physical properties
Particle weight 8times107 Diameter nm 30-80
Relative weght Composition (%) of Human Plasma Lipoproteins
Protein 10% Phospholipid 19% Free cholesterol 7.3% Cholesterol ester 4.6% Triglyceride 56%
Apolipoprotein contribution% apoB 37 apoC 50 apoE13
(Ref. 1301)
SPECTRAL DATA
UV SPECTRA:

IR SPECTRA:

NMR SPECTRA:

MASS SPECTRA:

OTHER SPECTRA:
CHROMATOGRAM DATA
VLDL on agarose gel electrophoresis
In agarose gel electrophoresis, VLDL migrate at the same position of preb-globulins, thus VLDL is designated preb-lipoprotein.
SOURCE

CHEMICAL SYNTHESIS
Structure of VLDL
VLDL is spherical particle and in normal individuals ranges 30 to 80 nm in diameter. The molecular weight is 10 to 8times107. Compare with LDL particles, VLDL particles are heterogeneous in size and composition. VLDL particls can be separated in Sf 20-60 and Sf60-400. VLDL consists of protein 10%, phospholipid 19%, free cholesterol 7%, cholesterol ester 10% and triglycerides 56%. Apolipoprotein contribution% apoB 37 apoC 50 apoE 13 Apo B-48 is the sole constituent apolipoprotein on VLDL.It is presumed that the cholesteryl esters and triglycerides form the hydrophobic core, which is surrounded by a surface coat of apolipoprotein B, free cholesterol and polar phospholipid components oriented toward the aqueous medium.
(Ref. 1301/1302/1303/1304/1305/1306)
METABOLISM
Metabolism of VLDL
VLDL is syncesized by the liver. VLDL is the major vehicle in the plasma which carries triacylglycerol synthesized in the liver to peripheral tissues for utilization. VLDL is catabolized by lipoprotein lipase. Concequently,VLDL is converted into IDL. The VLDL remnants are taken up into the liver via the LDL receptor and the LDL receptor-related protein.
(Ref. 1301/1307)
GENETIC INFORMATION

NOTE

REFERENCES
[1301]
AUTHOR:Gotto,A.M. Jr., Pownall,H.J. and Havel,R.L.
TITLE:Introduction to the Plasma Lipoproteins. PubMed ID:3523141
JOURNAL:Methods Enzymol.
VOL:128 PAGE : 3 -41 (1986)
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[1302]
AUTHOR:Alaupovic,P., Lee,D.M., and WcConathy, W.J.
TITLE: Studies on the Composition and Structure of Plasma Lipoproteins. Distribution of Lipoprotein Families in Major Density Classes of Normal Human Plasma Lipoproteins. PubMed ID:4337564
JOURNAL:Biochem.Biophys.Acta
VOL:260 PAGE : 689 -707 (1972)
[TOP]

[1303]
AUTHOR:Eisenberg,SSteiner,G. and Shafrir,E.
TITLE:Plasma Lipoproteins : Structure,Composition,Classification and Metabolism in Primary Hyperlipoproteinemias.
JOURNAL:McGraw-Hill
VOL: PAGE : 23 -41 (1991)
[TOP]

[1304]
AUTHOR:Eisenberg,S., Bilheimer,D.W., Levy,R.I.,and Lindgren,F.T.
TITLE:On the Metabolic Conversion of Human Plasma Very Low Density Lipoprotein to Low Density Lipoprotein. PubMed ID:4359865
JOURNAL:Biochem.Biophys.Acta
VOL:326 PAGE : 361 -377 (1973)
[TOP]

[1305]
AUTHOR:Eisenberg,S., and Levy,R.I.
TITLE:Lipoprotein Metabolism. PubMed ID:174409
JOURNAL:Adv Lipid Res.
VOL:13 PAGE : 1 -89 (1975)
[TOP]

[1306]
AUTHOR:Jacson,R.l.,Morrisett,J.D. and Gotto,A.M.Jr.
TITLE:Lipoprotein Structure and Metabolism.
JOURNAL:Physiol.Rev.
VOL:56 PAGE : 259 -316 (1976)
[TOP]

[1307]
AUTHOR:Janero, D. R., Siuta-Mangano, P., Miller, K. W., and Lane, M. D.
TITLE:Synthesis, processing, and secretion of hepatic very low density lipoprotein PubMed ID:6373801
JOURNAL:J Cell Biochem.
VOL:24 PAGE : 131-152 (1984)
[TOP]

Last updated June 19, 2007. Copyright © 1989-2007 Japanese Conference on the Biochemistry of Lipids (JCBL). All rights reserved.