Category:LBS/Protocol/Separation

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QAE-Sephadex (strong anion exchanger)
not adsorb (C/M/W fraction)
↓
acetylation 		adsorb (ammonium acetate fraction)
↓
Florisil (Mg silicate) DEAE-Sephadex (weak anion exchanger)
not adsorb
↓
neutral fraction
others 		adsorb
↓
twitterionic fraction
sphingomyelins 		not adsorb
↓
polar fraction
ceramide aminoetylphosphonate (CAEPn) 		adsorb
↓
acidic fraction
(sialic acids and uronate)
(sulfate and phosphate)

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