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=={{Bilingual|脂質分析の流れ|Analysis Flow}}==
=={{Bilingual|脂質分析の流れ|Analysis Flow}}==
# サンプルより脂質抽出
# Sample preparation :サンプルより脂質抽出
# 目的脂質の精製
# Analytical methods :解析方法、質量分析
# 液体クロマトグラフィー
# Idetification and multivariate analysis :同定と多変量解析
# マススペクトロメトリー
# 脂質の同定
# 多変量解析(主成分分析)


=={{Bilingual|論文一覧|Paper Summary}}==
=={{Bilingual|論文一覧|Paper Summary}}==

Revision as of 01:41, 1 October 2010

Lipidbase:メタボロミクス、特にリピドミクスにおける分析手法のデータベース

Lipidbase contains information of original papers that describe lipid analysis and identification. The database introduces useful methodologies in lipidomics for each lipid class such as acylglycerol, phospholipid, and glycolipid. Papers indexed here are classified for each experimental step: extraction, purification, liquid chromatography, mass spectrometry, and identification. The papers are also linked to PubMed database.

このデータベースは、脂質の分析から同定までが記載されている論文を掲載している。脂 質の網羅的・包括的解析に有用な分析手法をそれぞれの脂質(たとえばアシルグリセロ ール、リン脂質、糖脂質など)に分けて、抽出、精製、液体クロマトグラフィー、マスス ペクトロメトリー、脂質同定するまでの過程を簡単明瞭に理解できるようにした。詳細な 知識を得たいユーザーのために論文からPubMedの抄録のページへリンクを貼り、アクセ スを可能にした。

Contents

  • Paper Summary: 論文、分析した脂質、使用した機器名の一覧表 (このページ)
  • Lipid Class: 脂質略号一覧 (脂質名をクリックすると記述されている論文リストへ)
  • Analytical Methods: 分析略号一覧 (使用機器名をクリックすると記述されている論文リストへ)

Analysis Flow

  1.  Sample preparation :サンプルより脂質抽出
  2.  Analytical methods :解析方法、質量分析
  3.  Idetification and multivariate analysis :同定と多変量解析

Paper Summary

Total 16 original papers

paper ID authors title lipids MS LC
Taguchi:OP101
(PMID 17449863)

 
 
 
 
 
 
 

Changes in molecular species profiles of glycosylphosphatidylinositol anchor precursors in early stages of biosynthesis

 

 
 

 

Taguchi:OP104
(PMID 18206977)

 
 
 

Development of a reverse-phase liquid chromatography electrospray ionization mass spectrometry method for lipidomics, improving detection of phosphatidic acid and phosphatidylserine

 
 
 
 

 

 

Taguchi:OP110
(PMID 18703820)

 
 
 

Targeted analysis of ganglioside and sulfatide molecular species by LC/ESI-MS/MS with theoretically expanded multiple reaction monitoring

 
 
 
 
 
 
 
 
 
 
 

 

 

Taguchi:OP113
(PMID 18956880)

 
 

Reversed-Phase LC/MS Method for Polyphosphoinositide Analyses: Changes in Molecular Species Levels during Epidermal Growth Factor Activation in A431 Cells

 
 
 

 

 

Taguchi:OP114
(PMID 18964370)

 
 
 
 

Analysis of oxidized phosphatidylcholines as markers for oxidative stress, using multiple reaction monitoring with theoretically expanded data sets with reversed-phase liquid chromatography/tandem mass spectrometry

 

 

 
 

Taguchi:OP121
(PMID 19481987)

 
 
 
 

Global analysis of triacylglycerols including oxidized molecular species by reverse-phase high resolution LC/ESI-QTOF MS/MS

 
 

 

 

Taguchi:OP129
(PMID 19880374)

 
 
 
 

Separation and quantification of sn-1 and sn-2 fatty acid positional isomers in phosphatidylcholine by RPLC-ESIMS/MS

 
 

 

 

Taguchi:OP131
(PMID 20069690)

 
 
 
 
 

Liquid chromatography/mass spectrometry analysis revealing preferential occurrence of non-arachidonate-containing phosphatidylinositol bisphosphate species in nuclei and changes in their levels during cell cycle

 

 

 

Taguchi:OP134
(PMID 20452604)

 
 

Precise and global identification of phospholipid molecular species by an Orbitrap mass spectrometer and automated search engine Lipid Search

 
 
 
 
 
 

 

 

Taguchi:OP76
(PMID 10972995)

 
 
 
 

Two-dimensional analysis of phospholipids by capillary liquid chromatography/electrospray ionization mass spectrometry

 
 
 
 
 

 

 

Taguchi:OP87
(PMID 15384179)

 
 
 
 
 
 
 

High-resolution analysis by nano-electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry for the identification of molecular species of phospholipids and their oxidized metabolites

 
 
 
 

 
 

 

Taguchi:OP88
(PMID 15565732)

 
 
 
 
 
 

Rapid and selective identification of molecular species in phosphatidylcholine and sphingomyelin by conditional neutral loss scanning and MS3

 
 

 
 

 
 

Taguchi:OP90
(PMID )

 
 
 
 

Specific detection of lysophosphatidic acids in serum extracts by tandem mass spectrometry

 

 

Taguchi:OP91
(PMID 15700236)

 
 
 
 
 

A shotgun tandem mass spectrometric analysis of phospholipids with normal-phase and/or reverse-phase liquid chromatography/electrospray ionization mass spectrometry

 
 
 
 
 

 
 

 
 

Taguchi:OP93
(PMID 15990370)

 
 
 
 
 
 
 

Focused lipidomics by tandem mass spectrometry

 
 
 
 
 

 

 

Taguchi:OP94
(PMID 16407401)

 
 
 
 
 
 

PGAP2 Is Essential for Correct Processing and Stable Expression of GPI-anchored Proteins