Category:Method - Revision history

Editor: /* {{Bilingual|前処理|Sample Preparation}} */

2010-11-05T11:12:49Z

Dbmanager: /* {{Bilingual|前処理|Sample Preparation}} */

2010-10-26T08:50:34Z

← Older revision		Revision as of 17:50, 26 October 2010
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	** Chloroform-methanol extraction (Ledeen method) : [[:Category:Method/Sample/GL/Acidic\|Glycolipids(Gangliosides, Sulphatides)]]		** Chloroform-methanol extraction (Ledeen method) : [[:Category:Method/Sample/GL/Acidic\|Glycolipids(Gangliosides, Sulphatides)]]
	; Butanol extraction		; Butanol extraction
	** Solid-phase extraction using ion-exchange column DEAE-cellulose column [[:Category:Method/Sample/PL/Acidic\|Acidic PLs]]		** Solid-phase extraction using ion-exchange column DEAE-cellulose column [[:Category:Method/Sample/PL/Acidic\|Acidic PLs (PA, LPA, PS, LPS)]]
	\|		\|
	; 細胞・臓器をメタノールでホモジナイズ		; 細胞・臓器をメタノールでホモジナイズ

Dbmanager: /* {{Bilingual|前処理|Sample Preparation}} */

2010-10-26T08:49:12Z

← Older revision		Revision as of 17:49, 26 October 2010
Line 31:		Line 31:
	** Chloroform-methanol extraction (Ledeen method) : [[:Category:Method/Sample/GL/Acidic\|Glycolipids(Gangliosides, Sulphatides)]]		** Chloroform-methanol extraction (Ledeen method) : [[:Category:Method/Sample/GL/Acidic\|Glycolipids(Gangliosides, Sulphatides)]]
	; Butanol extraction		; Butanol extraction
	** Solid-phase~~, Ion~~-exchange column DEAE : [[:Category:Method/Sample/PL/Acidic\|Acidic PLs]]		** Solid-phase extraction using ion-exchange column DEAE-cellulose column [[:Category:Method/Sample/PL/Acidic\|Acidic PLs]]
	\|		\|
	; 細胞・臓器をメタノールでホモジナイズ		; 細胞・臓器をメタノールでホモジナイズ

Dbmanager: /* {{Bilingual|前処理|Sample Preparation}} */

2010-10-22T04:37:55Z

← Older revision		Revision as of 13:37, 22 October 2010
Line 25:		Line 25:
	{{Twocolumn\|		{{Twocolumn\|
	; Homogenize cells or organs with methanol		; Homogenize cells or organs with methanol
	** B&D method: [[:Category:Method/Sample/PL/Neutral\|Neutral lipids, ~~Phospholipid~~ (PLs)]]		** B&D method: [[:Category:Method/Sample/PL/Neutral\|Neutral lipids, Phospholipids (PLs)]]
	** Solid-phase extraction		** Solid-phase extraction
	*** Reverse column C18 : [[:Category:Method/Sample/PL/Oxidized\|Oxidized PLs, Oxidized fatty acids]]		*** Reverse column C18 : [[:Category:Method/Sample/PL/Oxidized\|Oxidized PLs, Oxidized fatty acids]]
	*** Ion-exchange column DEAE : [[:Category:Method/Sample/PL/Acidic_PI\|Phosphoinositides]]		*** Ion-exchange column DEAE : [[:Category:Method/Sample/PL/Acidic_PI\|Phosphoinositides]]
	** Chloroform-methanol extraction (Ledeen method) : [[:Category:Method/Sample/GL/Acidic\|~~Glycolipid~~(Gangliosides, Sulphatides)]]		** Chloroform-methanol extraction (Ledeen method) : [[:Category:Method/Sample/GL/Acidic\|Glycolipids(Gangliosides, Sulphatides)]]
	; Butanol extraction		; Butanol extraction
	** Solid-phase, Ion-exchange column DEAE : [[:Category:Method/Sample/PL/Acidic\|Acidic PLs]]		** Solid-phase, Ion-exchange column DEAE : [[:Category:Method/Sample/PL/Acidic\|Acidic PLs]]

Dbmanager: /* {{Bilingual|前処理|Sample Preparation}} */

2010-10-22T04:34:57Z

← Older revision		Revision as of 13:34, 22 October 2010
Line 29:		Line 29:
	*** Reverse column C18 : [[:Category:Method/Sample/PL/Oxidized\|Oxidized PLs, Oxidized fatty acids]]		*** Reverse column C18 : [[:Category:Method/Sample/PL/Oxidized\|Oxidized PLs, Oxidized fatty acids]]
	*** Ion-exchange column DEAE : [[:Category:Method/Sample/PL/Acidic_PI\|Phosphoinositides]]		*** Ion-exchange column DEAE : [[:Category:Method/Sample/PL/Acidic_PI\|Phosphoinositides]]
	** Chloroform-methanol extraction (Ledeen method) : [[:Category:Method/Sample/GL/Acidic\|~~Glycolipids~~, ~~Gangliosides~~]]		** Chloroform-methanol extraction (Ledeen method) : [[:Category:Method/Sample/GL/Acidic\|Glycolipid(Gangliosides, Sulphatides)]]
	; Butanol extraction		; Butanol extraction
	** Solid-phase, Ion-exchange column DEAE : [[:Category:Method/Sample/PL/Acidic\|Acidic PLs]]		** Solid-phase, Ion-exchange column DEAE : [[:Category:Method/Sample/PL/Acidic\|Acidic PLs]]
	\|		\|
	; 細胞・臓器をメタノールでホモジナイズ		; 細胞・臓器をメタノールでホモジナイズ
	** B&D法 : [[:Category:Method/Sample/PL/Neutral\|~~リン脂質、中性脂質~~]]		** B&D法 : [[:Category:Method/Sample/PL/Neutral\|中性脂質、リン脂質、]]
	** 固相抽出法		** 固相抽出法
	*** 逆相カラム（C18） : [[:Category:Method/Sample/PL/Oxidized\|酸化リン脂質、酸化脂肪酸]]		*** 逆相カラム（C18） : [[:Category:Method/Sample/PL/Oxidized\|酸化リン脂質、酸化脂肪酸]]
	*** イオン交換カラム（DEAEセルロース） : [[:Category:Method/Sample/PL/Acidic_PI\|ホスホイノシタイド		*** イオン交換カラム（DEAEセルロース） : [[:Category:Method/Sample/PL/Acidic_PI\|ホスホイノシタイド
	]]		]]
	** ~~クロメタ抽出法（Ledeen法の改変）~~ : [[:Category:Method/Sample/GL/Acidic\|~~糖脂質（ガングリオシド）~~]]		** クロロホルムーメタノール抽出法（Ledeen法の改変） : [[:Category:Method/Sample/GL/Acidic\|酸性糖脂質（ガングリオシド、スルファチド）]]
	; ブタノール抽出法		; ブタノール抽出法
	** イオン交換カラム（DEAEセルロース）を用いた固相抽出法 : [[:Category:Method/Sample/PL/Acidic\|酸性リン脂質（PA, LPA, PS, LPS）		** イオン交換カラム（DEAEセルロース）を用いた固相抽出法 : [[:Category:Method/Sample/PL/Acidic\|酸性リン脂質（PA, LPA, PS, LPS）

Dbmanager: /* {{Bilingual|分析|Analytical Strategy}} */

2010-10-22T03:03:11Z

← Older revision		Revision as of 12:03, 22 October 2010
Line 60:		Line 60:
	\|		\|
	''Hybrid type MS''		''Hybrid type MS''
	: {{Bilingual\|~~イオントラップ（IT）とTOFまたはFTのハイブリッド~~\| ion-trap (IT)-TOF or Fourier-Transform Ion Cyclotron Resonance (FT-ICR)}}		: {{Bilingual\|イオントラップ（IT）とTOFまたはフーリエ変換(FT)のハイブリッド\| ion-trap (IT)-TOF or Fourier-Transform Ion Cyclotron Resonance (FT-ICR)}}
	\|		\|
	* LTQ-Orbitrap (Theremo-Fisher Scientific)		* LTQ-Orbitrap (Theremo-Fisher Scientific)

Editor at 17:02, 15 October 2010

2010-10-15T17:02:29Z

← Older revision		Revision as of 02:02, 16 October 2010
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	# 数理解析　... 定性と定量ならびに多変量解析		# 数理解析　... 定性と定量ならびに多変量解析
	}}		}}
	\|		\|align="center"\|
	<small>'''{{Bilingual\|~~分子量と分子種~~\|Amount & Species}}'''<br/>		<small>'''{{Bilingual\|含有量と分子種\|Amount & Species}}'''<br/>
	{{Bilingual\|多い     少ない\|abundant      few}}<br/>		{{Bilingual\|多い     少ない\|abundant      few}}<br/>
	[[Image:LipidContents.png\|100px]]<br/>		[[Image:LipidContents.png\|100px]]<br/>
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	=={{Bilingual\|前処理\|Sample Preparation}}==		=={{Bilingual\|前処理\|Sample Preparation}}==
	{{Twocolumn\|		{{Twocolumn\|
	Sample ~~Preparation details for phospholipids~~ (PL) ~~and glycolipids (~~GL).		; Homogenize cells or organs with methanol
			** B&D method: [[:Category:Method/Sample/PL/Neutral\|Neutral lipids, Phospholipid (PLs)]]
			** Solid-phase extraction
			*** Reverse column C18 : [[:Category:Method/Sample/PL/Oxidized\|Oxidized PLs, Oxidized fatty acids]]
			*** Ion-exchange column DEAE : [[:Category:Method/Sample/PL/Acidic_PI\|Phosphoinositides]]
			** Chloroform-methanol extraction (Ledeen method) : [[:Category:Method/Sample/GL/Acidic\|Glycolipids, Gangliosides]]
			; Butanol extraction
			** Solid-phase, Ion-exchange column DEAE : [[:Category:Method/Sample/PL/Acidic\|Acidic PLs]]
	\|		\|
	~~リン脂質 (~~PL~~) および糖脂質 (GL) のサンプル調整法~~		; 細胞・臓器をメタノールでホモジナイズ
	~~}}{{#def~~:~~separator~~\|Sample/}}		** B&D法 : [[:Category:Method/Sample/PL/Neutral\|リン脂質、中性脂質]]
	~~{{#repeat~~:~~LinkCategoryPage\|1~~\|		** 固相抽出法
	~~{{#SearchTitle~~:Method/Sample\|~~Category}}~~		*** 逆相カラム（C18） : [[:Category:Method/Sample/PL/Oxidized\|酸化リン脂質、酸化脂肪酸]]
			*** イオン交換カラム（DEAEセルロース） : [[:Category:Method/Sample/PL/Acidic_PI\|ホスホイノシタイド
			]]
			** クロメタ抽出法（Ledeen法の改変） : [[:Category:Method/Sample/GL/Acidic\|糖脂質（ガングリオシド）]]
			; ブタノール抽出法
			** イオン交換カラム（DEAEセルロース）を用いた固相抽出法 : [[:Category:Method/Sample/PL/Acidic\|酸性リン脂質（PA, LPA, PS, LPS）
			]]
	}}		}}

	=={{Bilingual\|分析\|Analytical Strategy}}==		=={{Bilingual\|分析\|Analytical Strategy}}==
	{{~~#def~~:~~separator~~\|~~Analytical/~~}}
	{{~~#repeat:LinkCategoryPage~~\|1\|		{\| class="wikitable"
	{{~~#SearchTitle:Method~~/~~Analytical~~\|~~Category~~}}		! Strategy \|\| Method \|\| Type \|\| Instrument
	}}		\|-
			\|rowspan="2"\| '''Global''' ({{Bilingual\|ターゲットを絞らない網羅的手法\|detection of all lipids}})
			\|rowspan="2"\| LC/MS <br/> Data dependent scan (LC/MS/MS, LC/MS3)
			\|
			''Hybrid type MS''
			: {{Bilingual\|四重極（Q）と飛行時間（TOF）のハイブリッド\|quadrupole-time-of-flight (Q-TOF)}}
			\|
			* Q-TOF (Waters)
			\|-
			\|
			''Hybrid type MS''
			: {{Bilingual\|イオントラップ（IT）とTOFまたはFTのハイブリッド\| ion-trap (IT)-TOF or Fourier-Transform Ion Cyclotron Resonance (FT-ICR)}}
			\|
			* LTQ-Orbitrap (Theremo-Fisher Scientific)
			\|-
			\| '''Focused''' ({{Bilingual\|共通構造部分に焦点をあてた手法\|focusing on partial structures}})
			\| Precursor ion scan<br/> Neutral loss scan
			\|rowspan="2"\| {{Bilingual\|三連四重極（TSQ）MS
			\| Triple-quadrupole MS}}
			\|rowspan="2"\|
			* 4000 Q-TRAP or 5500 Q-TRAP (Applied Biosystems)
			* TSQ Quantum or Vantage (Thermo- Fisher Scientific)
			\|-
			\| '''Targeted''' ({{Bilingual\|個別分子特異的な手法\|targeting by molecular and fragment ions}})
			\| Multiple reaction monitoring (MRM),<br/> Selected reaction monitoring(SRM)
			\|}

	=={{Bilingual\|数理解析\|Computational Analysis}}==		=={{Bilingual\|数理解析\|Computational Analysis}}==

Editor at 16:22, 15 October 2010

2010-10-15T16:22:49Z

← Older revision		Revision as of 01:22, 16 October 2010
Line 1:		Line 1:
	{{Taguchi/Header}}		{{Taguchi/Header}}

	=={{Bilingual\|~~生体試料の調整~~\|Sample Preparation}}==		=={{Bilingual\|概要\|Overview}}==
			{\|
			\|
			{{Twocolumn\|
			Lipid contents exhibit high dynamic range. Detection of scarce elements is difficult as it is due to ion suppressions; different extraction methods are necessary depending on purposes.
			# Sample preparation ... extraction depending on purposes
			# Analytical strategy ... selection of platforms and methods
			# Computational analysis ... qualitative/quantitative and multivariate analysis
			\|
			脂質は量的に多い成分と少ない成分のダイナミックレンジが大きいため、主要成分と混合したままだと微量成分の検出はイオンサプレッションなどにより難しくなる。つまり、目的に応じたサンプル調製が重要である。
			# 前処理 ... 目的に応じたサンプル調製
			# 分析 ... 装置と測定方法の選択
			# 数理解析　... 定性と定量ならびに多変量解析
			}}
			\|
			<small>'''{{Bilingual\|分子量と分子種\|Amount & Species}}'''<br/>
			{{Bilingual\|多い     少ない\|abundant      few}}<br/>
			[[Image:LipidContents.png\|100px]]<br/>
			{{Bilingual\|少ない     多い\|scarce      many}}
			</small>
			\|}
			=={{Bilingual\|前処理\|Sample Preparation}}==
	{{Twocolumn\|		{{Twocolumn\|
	Sample Preparation details for phospholipids (PL) and glycolipids (GL).		Sample Preparation details for phospholipids (PL) and glycolipids (GL).
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	}}		}}

	=={{Bilingual\|~~分析法~~\|Analytical Strategy}}==		=={{Bilingual\|分析\|Analytical Strategy}}==
	{{#def:separator\|Analytical/}}		{{#def:separator\|Analytical/}}
	{{#repeat:LinkCategoryPage\|1\|		{{#repeat:LinkCategoryPage\|1\|
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	}}		}}

	=={{Bilingual\|~~数理的解析手法~~\|Computational ~~Methods~~}}==		=={{Bilingual\|数理解析\|Computational Analysis}}==

	* [http://lipidsearch.jp/ LipidSearch]		* [http://lipidsearch.jp/ LipidSearch]

Editor at 15:50, 15 October 2010

2010-10-15T15:50:59Z

← Older revision		Revision as of 00:50, 16 October 2010
Line 1:		Line 1:
			{{Taguchi/Header}}

	=={{Bilingual\|生体試料の調整\|Sample Preparation}}==		=={{Bilingual\|生体試料の調整\|Sample Preparation}}==
	{{Twocolumn\|		{{Twocolumn\|

Editor at 15:16, 15 October 2010

2010-10-15T15:16:51Z

← Older revision		Revision as of 00:16, 16 October 2010
Line 4:		Line 4:
	\|		\|
	リン脂質 (PL) および糖脂質 (GL) のサンプル調整法		リン脂質 (PL) および糖脂質 (GL) のサンプル調整法
	}}		}}{{#def:separator\|Sample/}}
	{{#def:separator\|Sample/}}
	{{#repeat:LinkCategoryPage\|1\|		{{#repeat:LinkCategoryPage\|1\|
	{{#SearchTitle:Method/Sample\|Category}}		{{#SearchTitle:Method/Sample\|Category}}
	}}		}}

	=={{Bilingual\|分析法\|Analytical ~~Category~~}}==		=={{Bilingual\|分析法\|Analytical Strategy}}==
	{{#def:separator\|Analytical/}}		{{#def:separator\|Analytical/}}
	{{#repeat:LinkCategoryPage\|1\|		{{#repeat:LinkCategoryPage\|1\|
	{{#SearchTitle:Method/Analytical\|Category}}		{{#SearchTitle:Method/Analytical\|Category}}
	}}		}}

			=={{Bilingual\|数理的解析手法\|Computational Methods}}==

			* [http://lipidsearch.jp/ LipidSearch]
			* [http://masspp.jp/ Mass++]
			* [http://www.infocom.co.jp/bio/develop/simca-p_plus.html SIMCA-P]
			* [http://www.absciex.com/ Marker View]
			* [http://www.thermoscientific.jp/lc-lcms/sieve.html SEIVE]
			* [http://www.ncc.go.jp/jp/nccri/divisions/03chem/pdf/2DICAL.pdf 2DICAL]

← Older revision		Revision as of 13:37, 22 October 2010
Line 25:		Line 25:
	{{Twocolumn\|		{{Twocolumn\|
	; Homogenize cells or organs with methanol		; Homogenize cells or organs with methanol
	** B&D method: [[:Category:Method/Sample/PL/Neutral\|Neutral lipids, ~~Phospholipid~~ (PLs)]]		** B&D method: [[:Category:Method/Sample/PL/Neutral\|Neutral lipids, Phospholipids (PLs)]]
	** Solid-phase extraction		** Solid-phase extraction
	*** Reverse column C18 : [[:Category:Method/Sample/PL/Oxidized\|Oxidized PLs, Oxidized fatty acids]]		*** Reverse column C18 : [[:Category:Method/Sample/PL/Oxidized\|Oxidized PLs, Oxidized fatty acids]]
	*** Ion-exchange column DEAE : [[:Category:Method/Sample/PL/Acidic_PI\|Phosphoinositides]]		*** Ion-exchange column DEAE : [[:Category:Method/Sample/PL/Acidic_PI\|Phosphoinositides]]
	** Chloroform-methanol extraction (Ledeen method) : [[:Category:Method/Sample/GL/Acidic\|~~Glycolipid~~(Gangliosides, Sulphatides)]]		** Chloroform-methanol extraction (Ledeen method) : [[:Category:Method/Sample/GL/Acidic\|Glycolipids(Gangliosides, Sulphatides)]]
	; Butanol extraction		; Butanol extraction
	** Solid-phase, Ion-exchange column DEAE : [[:Category:Method/Sample/PL/Acidic\|Acidic PLs]]		** Solid-phase, Ion-exchange column DEAE : [[:Category:Method/Sample/PL/Acidic\|Acidic PLs]]

← Older revision		Revision as of 13:34, 22 October 2010
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	*** Reverse column C18 : [[:Category:Method/Sample/PL/Oxidized\|Oxidized PLs, Oxidized fatty acids]]		*** Reverse column C18 : [[:Category:Method/Sample/PL/Oxidized\|Oxidized PLs, Oxidized fatty acids]]
	*** Ion-exchange column DEAE : [[:Category:Method/Sample/PL/Acidic_PI\|Phosphoinositides]]		*** Ion-exchange column DEAE : [[:Category:Method/Sample/PL/Acidic_PI\|Phosphoinositides]]
	** Chloroform-methanol extraction (Ledeen method) : [[:Category:Method/Sample/GL/Acidic\|~~Glycolipids~~, ~~Gangliosides~~]]		** Chloroform-methanol extraction (Ledeen method) : [[:Category:Method/Sample/GL/Acidic\|Glycolipid(Gangliosides, Sulphatides)]]
	; Butanol extraction		; Butanol extraction
	** Solid-phase, Ion-exchange column DEAE : [[:Category:Method/Sample/PL/Acidic\|Acidic PLs]]		** Solid-phase, Ion-exchange column DEAE : [[:Category:Method/Sample/PL/Acidic\|Acidic PLs]]
	\|		\|
	; 細胞・臓器をメタノールでホモジナイズ		; 細胞・臓器をメタノールでホモジナイズ
	** B&D法 : [[:Category:Method/Sample/PL/Neutral\|~~リン脂質、中性脂質~~]]		** B&D法 : [[:Category:Method/Sample/PL/Neutral\|中性脂質、リン脂質、]]
	** 固相抽出法		** 固相抽出法
	*** 逆相カラム（C18） : [[:Category:Method/Sample/PL/Oxidized\|酸化リン脂質、酸化脂肪酸]]		*** 逆相カラム（C18） : [[:Category:Method/Sample/PL/Oxidized\|酸化リン脂質、酸化脂肪酸]]
	*** イオン交換カラム（DEAEセルロース） : [[:Category:Method/Sample/PL/Acidic_PI\|ホスホイノシタイド		*** イオン交換カラム（DEAEセルロース） : [[:Category:Method/Sample/PL/Acidic_PI\|ホスホイノシタイド
	]]		]]
	** ~~クロメタ抽出法（Ledeen法の改変）~~ : [[:Category:Method/Sample/GL/Acidic\|~~糖脂質（ガングリオシド）~~]]		** クロロホルムーメタノール抽出法（Ledeen法の改変） : [[:Category:Method/Sample/GL/Acidic\|酸性糖脂質（ガングリオシド、スルファチド）]]
	; ブタノール抽出法		; ブタノール抽出法
	** イオン交換カラム（DEAEセルロース）を用いた固相抽出法 : [[:Category:Method/Sample/PL/Acidic\|酸性リン脂質（PA, LPA, PS, LPS）		** イオン交換カラム（DEAEセルロース）を用いた固相抽出法 : [[:Category:Method/Sample/PL/Acidic\|酸性リン脂質（PA, LPA, PS, LPS）

← Older revision		Revision as of 02:02, 16 October 2010
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	# 数理解析　... 定性と定量ならびに多変量解析		# 数理解析　... 定性と定量ならびに多変量解析
	}}		}}
	\|		\|align="center"\|
	<small>'''{{Bilingual\|~~分子量と分子種~~\|Amount & Species}}'''<br/>		<small>'''{{Bilingual\|含有量と分子種\|Amount & Species}}'''<br/>
	{{Bilingual\|多い     少ない\|abundant      few}}<br/>		{{Bilingual\|多い     少ない\|abundant      few}}<br/>
	[[Image:LipidContents.png\|100px]]<br/>		[[Image:LipidContents.png\|100px]]<br/>
Line 24:		Line 24:
	=={{Bilingual\|前処理\|Sample Preparation}}==		=={{Bilingual\|前処理\|Sample Preparation}}==
	{{Twocolumn\|		{{Twocolumn\|
	Sample ~~Preparation details for phospholipids~~ (PL) ~~and glycolipids (~~GL).		; Homogenize cells or organs with methanol
			** B&D method: [[:Category:Method/Sample/PL/Neutral\|Neutral lipids, Phospholipid (PLs)]]
			** Solid-phase extraction
			*** Reverse column C18 : [[:Category:Method/Sample/PL/Oxidized\|Oxidized PLs, Oxidized fatty acids]]
			*** Ion-exchange column DEAE : [[:Category:Method/Sample/PL/Acidic_PI\|Phosphoinositides]]
			** Chloroform-methanol extraction (Ledeen method) : [[:Category:Method/Sample/GL/Acidic\|Glycolipids, Gangliosides]]
			; Butanol extraction
			** Solid-phase, Ion-exchange column DEAE : [[:Category:Method/Sample/PL/Acidic\|Acidic PLs]]
	\|		\|
	~~リン脂質 (~~PL~~) および糖脂質 (GL) のサンプル調整法~~		; 細胞・臓器をメタノールでホモジナイズ
	~~}}{{#def~~:~~separator~~\|Sample/}}		** B&D法 : [[:Category:Method/Sample/PL/Neutral\|リン脂質、中性脂質]]
	~~{{#repeat~~:~~LinkCategoryPage\|1~~\|		** 固相抽出法
	~~{{#SearchTitle~~:Method/Sample\|~~Category}}~~		*** 逆相カラム（C18） : [[:Category:Method/Sample/PL/Oxidized\|酸化リン脂質、酸化脂肪酸]]
			*** イオン交換カラム（DEAEセルロース） : [[:Category:Method/Sample/PL/Acidic_PI\|ホスホイノシタイド
			]]
			** クロメタ抽出法（Ledeen法の改変） : [[:Category:Method/Sample/GL/Acidic\|糖脂質（ガングリオシド）]]
			; ブタノール抽出法
			** イオン交換カラム（DEAEセルロース）を用いた固相抽出法 : [[:Category:Method/Sample/PL/Acidic\|酸性リン脂質（PA, LPA, PS, LPS）
			]]
	}}		}}

	=={{Bilingual\|分析\|Analytical Strategy}}==		=={{Bilingual\|分析\|Analytical Strategy}}==
	{{~~#def~~:~~separator~~\|~~Analytical/~~}}
	{{~~#repeat:LinkCategoryPage~~\|1\|		{\| class="wikitable"
	{{~~#SearchTitle:Method~~/~~Analytical~~\|~~Category~~}}		! Strategy \|\| Method \|\| Type \|\| Instrument
	}}		\|-
			\|rowspan="2"\| '''Global''' ({{Bilingual\|ターゲットを絞らない網羅的手法\|detection of all lipids}})
			\|rowspan="2"\| LC/MS <br/> Data dependent scan (LC/MS/MS, LC/MS3)
			\|
			''Hybrid type MS''
			: {{Bilingual\|四重極（Q）と飛行時間（TOF）のハイブリッド\|quadrupole-time-of-flight (Q-TOF)}}
			\|
			* Q-TOF (Waters)
			\|-
			\|
			''Hybrid type MS''
			: {{Bilingual\|イオントラップ（IT）とTOFまたはFTのハイブリッド\| ion-trap (IT)-TOF or Fourier-Transform Ion Cyclotron Resonance (FT-ICR)}}
			\|
			* LTQ-Orbitrap (Theremo-Fisher Scientific)
			\|-
			\| '''Focused''' ({{Bilingual\|共通構造部分に焦点をあてた手法\|focusing on partial structures}})
			\| Precursor ion scan<br/> Neutral loss scan
			\|rowspan="2"\| {{Bilingual\|三連四重極（TSQ）MS
			\| Triple-quadrupole MS}}
			\|rowspan="2"\|
			* 4000 Q-TRAP or 5500 Q-TRAP (Applied Biosystems)
			* TSQ Quantum or Vantage (Thermo- Fisher Scientific)
			\|-
			\| '''Targeted''' ({{Bilingual\|個別分子特異的な手法\|targeting by molecular and fragment ions}})
			\| Multiple reaction monitoring (MRM),<br/> Selected reaction monitoring(SRM)
			\|}

	=={{Bilingual\|数理解析\|Computational Analysis}}==		=={{Bilingual\|数理解析\|Computational Analysis}}==

← Older revision		Revision as of 20:12, 5 November 2010
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	=={{Bilingual\|前処理\|Sample Preparation}}==		=={{Bilingual\|前処理\|Sample Preparation}}==
	{{Twocolumn\|		{{Twocolumn\|
	; Homogenize cells or organs with methanol		; Strategy 1. Homogenize cells or organs with methanol
	** B&D method: [[:Category:Method/Sample/PL/Neutral\|Neutral lipids, Phospholipids (PLs)]]		# B&D method: [[:Category:Method/Sample/PL/Neutral\|Neutral lipids, Phospholipids (PLs)]]
	** Solid-phase extraction		# Solid-phase extraction
	~~***~~ Reverse column C18 : [[:Category:Method/Sample/PL/Oxidized\|Oxidized PLs, Oxidized fatty acids]]		## Reverse column C18 : [[:Category:Method/Sample/PL/Oxidized\|Oxidized PLs, Oxidized fatty acids]]
	~~***~~ Ion-exchange column DEAE : [[:Category:Method/Sample/PL/Acidic_PI\|Phosphoinositides]]		## Ion-exchange column DEAE : [[:Category:Method/Sample/PL/Acidic_PI\|Phosphoinositides]]
	** Chloroform-methanol extraction (Ledeen method) : [[:Category:Method/Sample/GL/Acidic\|Glycolipids(Gangliosides, Sulphatides)]]		# Chloroform-methanol extraction (Ledeen method) : [[:Category:Method/Sample/GL/Acidic\|Glycolipids(Gangliosides, Sulphatides)]]
	; Butanol extraction		; Strategy 2. Butanol extraction
	** Solid-phase extraction using ion-exchange column DEAE-cellulose column [[:Category:Method/Sample/PL/Acidic\|Acidic PLs (PA, LPA, PS, LPS)]]		# Solid-phase extraction using ion-exchange column DEAE-cellulose column [[:Category:Method/Sample/PL/Acidic\|Acidic PLs (PA, LPA, PS, LPS)]]
	\|		\|
	; 細胞・臓器をメタノールでホモジナイズ		;手法その1 細胞・臓器をメタノールでホモジナイズ
	** B&D法 : [[:Category:Method/Sample/PL/Neutral\|中性脂質、リン脂質、]]		# B&D法 : [[:Category:Method/Sample/PL/Neutral\|中性脂質、リン脂質、]]
	** 固相抽出法		# 固相抽出法
	~~***~~ 逆相カラム（C18） : [[:Category:Method/Sample/PL/Oxidized\|酸化リン脂質、酸化脂肪酸]]		## 逆相カラム（C18） : [[:Category:Method/Sample/PL/Oxidized\|酸化リン脂質、酸化脂肪酸]]
	~~***~~ イオン交換カラム（DEAEセルロース） : [[:Category:Method/Sample/PL/Acidic_PI\|ホスホイノシタイド		## イオン交換カラム（DEAEセルロース） : [[:Category:Method/Sample/PL/Acidic_PI\|ホスホイノシタイド
	]]		]]
	** クロロホルムーメタノール抽出法（Ledeen法の改変） : [[:Category:Method/Sample/GL/Acidic\|酸性糖脂質（ガングリオシド、スルファチド）]]		# クロロホルムーメタノール抽出法（Ledeen法の改変） : [[:Category:Method/Sample/GL/Acidic\|酸性糖脂質（ガングリオシド、スルファチド）]]
	; ブタノール抽出法		;手法その2 ブタノール抽出法
	** イオン交換カラム（DEAEセルロース）を用いた固相抽出法 : [[:Category:Method/Sample/PL/Acidic\|酸性リン脂質（PA, LPA, PS, LPS）		# イオン交換カラム（DEAEセルロース）を用いた固相抽出法 : [[:Category:Method/Sample/PL/Acidic\|酸性リン脂質（PA, LPA, PS, LPS）
	]]		]]
	}}		}}