Category:Method/Sample/GL/Acidic - Revision history

Editor at 08:17, 5 November 2010

2010-11-05T08:17:38Z

← Older revision		Revision as of 17:17, 5 November 2010
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			{{Taguchi/Header}}
	=Acidic glycolipids (gangliosides, sulphatides)=		=Acidic glycolipids (gangliosides, sulphatides)=

Dbmanager at 06:54, 22 October 2010

2010-10-22T06:54:25Z

← Older revision		Revision as of 15:54, 22 October 2010
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	# Acidic glycolipid mixtures were eluted with 7.5 ml chloroform-methanol-0.8 M sodium acetate (30:60:8; v/v/v).		# Acidic glycolipid mixtures were eluted with 7.5 ml chloroform-methanol-0.8 M sodium acetate (30:60:8; v/v/v).
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	# マウス脳　(500 mg)　からクロロホルム:メタノール混液 (2:1, v/v) 9 ml, クロロホルム:メタノール:水混液 (1:2:0.8, v/v/v) 7.6 ml ~~で抽出~~		# マウス脳　(500 mg)　からクロロホルム:メタノール混液 (2:1, v/v) 9 ml, クロロホルム:メタノール:水混液 (1:2:0.8, v/v/v) 7.6 ml で脂質を抽出
	# 窒素ガス下で乾固、クロロホルム:メタノール:水混液 (30:60:8; v/v/v) 7.5 ml に再度溶解		# 窒素ガス下で乾固、クロロホルム:メタノール:水混液 (30:60:8; v/v/v) 7.5 ml に再度溶解
	# DEAE-Sephadex A-25 カラム (6 x 45 mm, acetate form) (Amersham Biosciences AB, Sweden) ~~にセット~~		# DEAE-Sephadex A-25 カラム (6 x 45 mm, acetate form) (Amersham Biosciences AB, Sweden) に注入
	# 中性脂質をクロロホルム:メタノール:水混合 (30:60:8; v/v/v) 15 ml で溶出		# 中性脂質をクロロホルム:メタノール:水混合 (30:60:8; v/v/v) 15 ml で溶出
	# ~~酸性の糖脂質混合物を、~~ クロロホルム:メタノール:0.8M 酢酸ナトリウム混液 (30:60:8; v/v/v) 7.5 ml で溶出		# 酸性糖脂質を、クロロホルム:メタノール:0.8M 酢酸ナトリウム混液 (30:60:8; v/v/v) 7.5 ml で溶出
	}}		}}

Dbmanager at 06:52, 22 October 2010

2010-10-22T06:52:26Z

← Older revision		Revision as of 15:52, 22 October 2010
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	# Acidic glycolipid mixtures were eluted with 7.5 ml chloroform-methanol-0.8 M sodium acetate (30:60:8; v/v/v).		# Acidic glycolipid mixtures were eluted with 7.5 ml chloroform-methanol-0.8 M sodium acetate (30:60:8; v/v/v).
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	# マウス脳　(500 mg)　からクロロホルム:メタノール混液 (2:1, v/v)9 ml, クロロホルム:メタノール:水混液 (1:2:0.8, v/v/v) 7.6 ml で抽出		# マウス脳　(500 mg)　からクロロホルム:メタノール混液 (2:1, v/v) 9 ml, クロロホルム:メタノール:水混液 (1:2:0.8, v/v/v) 7.6 ml で抽出
	# ~~ゆるやかな窒素ガスで乾燥、7.5 ml クロロホルム~~:メタノール:~~水混合~~ (30:60:8; v/v/v) に再度溶解		# 窒素ガス下で乾固、クロロホルム:メタノール:水混液 (30:60:8; v/v/v) 7.5 ml に再度溶解
	# DEAE-Sephadex A-25 カラム (6 x 45 mm, acetate form) (Amersham Biosciences AB, Sweden) にセット		# DEAE-Sephadex A-25 カラム (6 x 45 mm, acetate form) (Amersham Biosciences AB, Sweden) にセット
	# 中性脂質を ~~15 ml~~ クロロホルム:メタノール:水混合 (30:60:8; v/v/v) ~~で洗浄~~		# 中性脂質をクロロホルム:メタノール:水混合 (30:60:8; v/v/v) 15 ml で溶出
	# 酸性の糖脂質混合物を、 ~~7.5 ml~~ クロロホルム:メタノール:0.8M ~~酢酸ナトリウム混合~~ (30:60:8; v/v/v) で溶出		# 酸性の糖脂質混合物を、クロロホルム:メタノール:0.8M 酢酸ナトリウム混液 (30:60:8; v/v/v) 7.5 ml で溶出
	}}		}}

Dbmanager at 06:48, 22 October 2010

2010-10-22T06:48:34Z

← Older revision		Revision as of 15:48, 22 October 2010
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	{{Twocolumn\|		{{Twocolumn\|
	# Lipids were extracted from mouse brain with 9 ml chloroform–methanol (2:1, v/v) and then with 7.6 ml chloroform–methanol-water (1:2:0.8, v/v/v).		# Lipids were extracted from mouse brain (500 mg) with 9 ml chloroform–methanol (2:1, v/v) and then with 7.6 ml chloroform–methanol-water (1:2:0.8, v/v/v).
	# The extraction was dried under a gentle stream of nitrogen and re-dissolved with 7.5 ml chloroform-methanol-water(30:60:8; v/v/v).		# The extraction was dried under a gentle stream of nitrogen and re-dissolved with 7.5 ml chloroform-methanol-water(30:60:8; v/v/v).
	# Samples were applied to a DEAE-Sephadex A-25 column (6 x 45 mm, acetate form) (Amersham Biosciences AB, Sweden).		# Samples were applied to a DEAE-Sephadex A-25 column (6 x 45 mm, acetate form) (Amersham Biosciences AB, Sweden).

Dbmanager at 06:47, 22 October 2010

2010-10-22T06:47:30Z

← Older revision		Revision as of 15:47, 22 October 2010
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	{{Twocolumn\|		{{Twocolumn\|
	# Lipids were extracted from mouse brain with chloroform–methanol (2:1, v/v) and then with chloroform–methanol-water (1:2:0.8, v/v/v).		# Lipids were extracted from mouse brain with 9 ml chloroform–methanol (2:1, v/v) and then with 7.6 ml chloroform–methanol-water (1:2:0.8, v/v/v).
	# The extraction was dried under a gentle stream of nitrogen and re-dissolved with 7.5 ml chloroform-methanol-water(30:60:8; v/v/v).		# The extraction was dried under a gentle stream of nitrogen and re-dissolved with 7.5 ml chloroform-methanol-water(30:60:8; v/v/v).
	# Samples were applied to a DEAE-Sephadex A-25 column (6 x 45 mm, acetate form) (Amersham Biosciences AB, Sweden).		# Samples were applied to a DEAE-Sephadex A-25 column (6 x 45 mm, acetate form) (Amersham Biosciences AB, Sweden).
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	# Acidic glycolipid mixtures were eluted with 7.5 ml chloroform-methanol-0.8 M sodium acetate (30:60:8; v/v/v).		# Acidic glycolipid mixtures were eluted with 7.5 ml chloroform-methanol-0.8 M sodium acetate (30:60:8; v/v/v).
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	# ~~クロロホルム~~:~~メタノール混合~~ (2:1, v/v), クロロホルム:メタノール:~~水混合~~ (1:2:0.8, v/v/v) で抽出		# マウス脳　(500 mg)　からクロロホルム:メタノール混液 (2:1, v/v)9 ml, クロロホルム:メタノール:水混液 (1:2:0.8, v/v/v) 7.6 ml で抽出
	# ゆるやかな窒素ガスで乾燥、7.5 ml クロロホルム:メタノール:水混合 (30:60:8; v/v/v) に再度溶解		# ゆるやかな窒素ガスで乾燥、7.5 ml クロロホルム:メタノール:水混合 (30:60:8; v/v/v) に再度溶解
	# DEAE-Sephadex A-25 カラム (6 x 45 mm, acetate form) (Amersham Biosciences AB, Sweden) にセット		# DEAE-Sephadex A-25 カラム (6 x 45 mm, acetate form) (Amersham Biosciences AB, Sweden) にセット

Dbmanager: /* Acidic glycolipids (gangliosides, sulphatides) */

2010-10-22T06:44:45Z

Acidic glycolipids (gangliosides, sulphatides)

← Older revision		Revision as of 15:44, 22 October 2010
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	{{Twocolumn\|		{{Twocolumn\|
	# Lipids were extracted with chloroform–methanol (2:1, v/v) and chloroform–methanol-water (1:2:0.8, v/v/v).		# Lipids were extracted from mouse brain with chloroform–methanol (2:1, v/v) and then with chloroform–methanol-water (1:2:0.8, v/v/v).
	# The extraction was dried under a gentle stream of nitrogen and re-dissolved with 7.5 ml chloroform-methanol-water(30:60:8; v/v/v).		# The extraction was dried under a gentle stream of nitrogen and re-dissolved with 7.5 ml chloroform-methanol-water(30:60:8; v/v/v).
	# Samples were applied to a DEAE-Sephadex A-25 column (6 x 45 mm, acetate form) (Amersham Biosciences AB, Sweden).		# Samples were applied to a DEAE-Sephadex A-25 column (6 x 45 mm, acetate form) (Amersham Biosciences AB, Sweden).
	# ~~Wash neutral~~ lipids ~~from the column~~ with 15 ml chloroform-methanol-water (30:60:8; v/v/v)		# Neutral lipids were eluted with 15 ml chloroform-methanol-water (30:60:8; v/v/v).
	# Acidic glycolipid mixtures were eluted with 7.5 ml chloroform-methanol-0.8 M sodium acetate (30:60:8; v/v/v).		# Acidic glycolipid mixtures were eluted with 7.5 ml chloroform-methanol-0.8 M sodium acetate (30:60:8; v/v/v).
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Editor: New page: =Acidic glycolipids (gangliosides, sulphatides)= {{Twocolumn| # Lipids were extracted with chloroform–methanol (2:1, v/v) and chloroform–methanol-water (1:2:0.8, v/v/v). # The extrac...

2010-10-10T01:26:01Z

New page: =Acidic glycolipids (gangliosides, sulphatides)= {{Twocolumn| # Lipids were extracted with chloroform–methanol (2:1, v/v) and chloroform–methanol-water (1:2:0.8, v/v/v). # The extrac...

New page

=Acidic glycolipids (gangliosides, sulphatides)=

{{Twocolumn|
# Lipids were extracted with chloroform–methanol (2:1, v/v) and chloroform–methanol-water (1:2:0.8, v/v/v).
# The extraction was dried under a gentle stream of nitrogen and re-dissolved with 7.5 ml chloroform-methanol-water(30:60:8; v/v/v).
# Samples were applied to a DEAE-Sephadex A-25 column (6 x 45 mm, acetate form) (Amersham Biosciences AB, Sweden).
# Wash neutral lipids from the column with 15 ml chloroform-methanol-water (30:60:8; v/v/v)
# Acidic glycolipid mixtures were eluted with 7.5 ml chloroform-methanol-0.8 M sodium acetate (30:60:8; v/v/v).
|
# クロロホルム:メタノール混合 (2:1, v/v), クロロホルム:メタノール:水混合 (1:2:0.8, v/v/v) で抽出
# ゆるやかな窒素ガスで乾燥、7.5 ml クロロホルム:メタノール:水混合 (30:60:8; v/v/v) に再度溶解
# DEAE-Sephadex A-25 カラム (6 x 45 mm, acetate form) (Amersham Biosciences AB, Sweden) にセット
# 中性脂質を 15 ml クロロホルム:メタノール:水混合 (30:60:8; v/v/v) で洗浄
# 酸性の糖脂質混合物を、 7.5 ml クロロホルム:メタノール:0.8M 酢酸ナトリウム混合 (30:60:8; v/v/v) で溶出
}}