LBGPEccp:R:R:YS2ANe005 - Revision history

Editor: LBGPEccp:R:R:YS2ANe005:01 moved to LBGPEccp:R:R:YS2ANe005

2010-04-14T21:00:00Z

LBGPEccp:R:R:YS2ANe005:01 moved to LBGPEccp:R:R:YS2ANe005

← Older revision		Revision as of 06:00, 15 April 2010
Line 4:		Line 4:
	\|LipidBank=PGP2410		\|LipidBank=PGP2410
	\|SysName=L-a-phosphatidylethanolamine		\|SysName=L-a-phosphatidylethanolamine
	\|Common Name=&&phosphatidylethanolamine&& cephalin&& ethanolaminephosphoglyceride&& (3-sn-phosphatidyl) ethanolamine ~~&&L-a-phosphatidylethanolamine~~&&		\|Common Name=&&phosphatidylethanolamine&&cephalin&&ethanolaminephosphoglyceride&&(3-sn-phosphatidyl) ethanolamine&&
	\|Melting Point=196°C <<2455>>; dimyristoyl-PE, 175-177°C <<2456/2457>>; dipalmitoyl-PE, 172-175°C <<2456/2457>> 210-211°C <<2469>> dipalmitoyl-DL-, 211°C <<2469>>; distearoyl-PE, 172-173.5°C <<2456/2457>>; dioleoyl-PE, 195-200°C <<2469>>; dielaidoyl-PE, 193°C <<2469>>; 1-palmitoyl-2-oleoyl-PE, 194-196°C <<2471>>		\|Melting Point=196°C <<2455>>; dimyristoyl-PE, 175-177°C <<2456/2457>>; dipalmitoyl-PE, 172-175°C <<2456/2457>> 210-211°C <<2469>> dipalmitoyl-DL-, 211°C <<2469>>; distearoyl-PE, 172-173.5°C <<2456/2457>>; dioleoyl-PE, 195-200°C <<2469>>; dielaidoyl-PE, 193°C <<2469>>; 1-palmitoyl-2-oleoyl-PE, 194-196°C <<2471>>
	\|Reflactive=dimyristoyl-PE , [ alpha ]^{26}_D +6.7° in CHCl_3 <<2456/2457>>; dipalmitoyl-PE, [ alpha ]^{26}_D +6.4° in CHCl_3 <<2456/2457>>, [ alpha ]^{25}_D +6.2° in CHCl_3 -MeOH (2:1,v/v) <<2469>>; distearoyl-PE, [ alpha ]^{24}_D +6.0° in CHCl_3 -glacial acetic acid (9:1,v/v) <<2456/2457>>; dioleoyl-PE, [ alpha ]^{22}_D +6.0° in CHCl_3 <<2469>>; dielaidoyl-PE, [ alpha ]^{22}_D +6.1° in CHCl_3 <<2469>>; 1-palmitoyl-2-oleoyl-PE, [ alpha ]^{22}_D +6.35° in CHCl_3 <<2469>>		\|Reflactive=dimyristoyl-PE , [ alpha ]^{26}_D +6.7° in CHCl_3 <<2456/2457>>; dipalmitoyl-PE, [ alpha ]^{26}_D +6.4° in CHCl_3 <<2456/2457>>, [ alpha ]^{25}_D +6.2° in CHCl_3 -MeOH (2:1,v/v) <<2469>>; distearoyl-PE, [ alpha ]^{24}_D +6.0° in CHCl_3 -glacial acetic acid (9:1,v/v) <<2456/2457>>; dioleoyl-PE, [ alpha ]^{22}_D +6.0° in CHCl_3 <<2469>>; dielaidoyl-PE, [ alpha ]^{22}_D +6.1° in CHCl_3 <<2469>>; 1-palmitoyl-2-oleoyl-PE, [ alpha ]^{22}_D +6.35° in CHCl_3 <<2469>>

Editor: LBGPEccp:R:R:YS2ANe005:01 moved to LBGPEccp:R:R:YS2ANe005

2010-02-25T10:00:10Z

LBGPEccp:R:R:YS2ANe005:01 moved to LBGPEccp:R:R:YS2ANe005

← Older revision		Revision as of 19:00, 25 February 2010
Line 6:		Line 6:
	\|Common Name=&&phosphatidylethanolamine&& cephalin&& ethanolaminephosphoglyceride&& (3-sn-phosphatidyl) ethanolamine &&L-a-phosphatidylethanolamine&&		\|Common Name=&&phosphatidylethanolamine&& cephalin&& ethanolaminephosphoglyceride&& (3-sn-phosphatidyl) ethanolamine &&L-a-phosphatidylethanolamine&&
	\|Melting Point=196°C <<2455>>; dimyristoyl-PE, 175-177°C <<2456/2457>>; dipalmitoyl-PE, 172-175°C <<2456/2457>> 210-211°C <<2469>> dipalmitoyl-DL-, 211°C <<2469>>; distearoyl-PE, 172-173.5°C <<2456/2457>>; dioleoyl-PE, 195-200°C <<2469>>; dielaidoyl-PE, 193°C <<2469>>; 1-palmitoyl-2-oleoyl-PE, 194-196°C <<2471>>		\|Melting Point=196°C <<2455>>; dimyristoyl-PE, 175-177°C <<2456/2457>>; dipalmitoyl-PE, 172-175°C <<2456/2457>> 210-211°C <<2469>> dipalmitoyl-DL-, 211°C <<2469>>; distearoyl-PE, 172-173.5°C <<2456/2457>>; dioleoyl-PE, 195-200°C <<2469>>; dielaidoyl-PE, 193°C <<2469>>; 1-palmitoyl-2-oleoyl-PE, 194-196°C <<2471>>
	\|Reflactive=dimyristoyl-PE , [ alpha ]^~~26_D~~ +6.7° in CHCl_3 <<2456/2457>>; dipalmitoyl-PE, [ alpha ]^~~26_D~~ +6.4° in CHCl_3 <<2456/2457>>, [ alpha ]^~~25_D~~ +6.2° in CHCl_3 -MeOH (2:1,v/v) <<2469>>; distearoyl-PE, [ alpha ]^~~24_D~~ +6.0° in CHCl_3 -glacial acetic acid (9:1,v/v) <<2456/2457>>; dioleoyl-PE, [ alpha ]^~~22_D~~ +6.0° in CHCl_3 <<2469>>; dielaidoyl-PE, [ alpha ]^~~22_D~~ +6.1° in CHCl_3 <<2469>>; 1-palmitoyl-2-oleoyl-PE, [ alpha ]^~~22_D~~ +6.35° in CHCl_3 <<2469>>		\|Reflactive=dimyristoyl-PE , [ alpha ]^{26}_D +6.7° in CHCl_3 <<2456/2457>>; dipalmitoyl-PE, [ alpha ]^{26}_D +6.4° in CHCl_3 <<2456/2457>>, [ alpha ]^{25}_D +6.2° in CHCl_3 -MeOH (2:1,v/v) <<2469>>; distearoyl-PE, [ alpha ]^{24}_D +6.0° in CHCl_3 -glacial acetic acid (9:1,v/v) <<2456/2457>>; dioleoyl-PE, [ alpha ]^{22}_D +6.0° in CHCl_3 <<2469>>; dielaidoyl-PE, [ alpha ]^{22}_D +6.1° in CHCl_3 <<2469>>; 1-palmitoyl-2-oleoyl-PE, [ alpha ]^{22}_D +6.35° in CHCl_3 <<2469>>
	\|Solubility=Insoluble in water. Natural PE is soluble in MeOH, EtOH, CHCl_3 , benzene, ether, and petroleum ether, and insoluble in acetone. At 20°C, synthetic PEs are insoluble (<=1mg/100 ml of dry solvent) in acetone, ether, petroleum ether, and ethyl acetate; moderately soluble (20-100 mg/100 ml of dry solvent) in EtOH, pyridine, benzene, and CCl_4 ; readily soluble (> 1g/100 ml of solvent) in CHCl_3 <<2455/2456/2457>>.		\|Solubility=Insoluble in water. Natural PE is soluble in MeOH, EtOH, CHCl_3 , benzene, ether, and petroleum ether, and insoluble in acetone. At 20°C, synthetic PEs are insoluble (<=1mg/100 ml of dry solvent) in acetone, ether, petroleum ether, and ethyl acetate; moderately soluble (20-100 mg/100 ml of dry solvent) in EtOH, pyridine, benzene, and CCl_4 ; readily soluble (> 1g/100 ml of solvent) in CHCl_3 <<2455/2456/2457>>.
	\|Mass Spectra=Negative ion mass spectrum during the elution of 1-stearol-2-arachidonyl-PE in a sample of rat liver PE. A Fisons VG Platform II single quadrupole mass spectrometer, fitted with an electrospray ion source operated at atmospheric pressure, was used for the identification of components eluting from the column. Nitrogen was used as nebulizer gas and as curtain gas. The capillary voltage was set to 3.0 kV and the cone voltage to 100 V {{Image200\|LBGPEccp:R:R:YS2ANe005SP0004.gif}} <<2462>>.,		\|Mass Spectra=Negative ion mass spectrum during the elution of 1-stearol-2-arachidonyl-PE in a sample of rat liver PE. A Fisons VG Platform II single quadrupole mass spectrometer, fitted with an electrospray ion source operated at atmospheric pressure, was used for the identification of components eluting from the column. Nitrogen was used as nebulizer gas and as curtain gas. The capillary voltage was set to 3.0 kV and the cone voltage to 100 V {{Image200\|LBGPEccp:R:R:YS2ANe005SP0004.gif}} <<2462>>.,
	\|UV Spectra=Maximum absorbance at 205 nm <<2458>>.		\|UV Spectra=Maximum absorbance at 205 nm <<2458>>.
	\|IR Spectra=Fourier transform IR spectra for dimyristoyl-PE in the dry state (anhydrous), either in the pure state (A, C and E) or in the presence of 20 mol% of alpha -tocopherol (B, D and F). Ester C=O stretching vibration mode region,1800-1700 cm^- ^1 ; ~~CH_2~~^- ^1 ; headgroup ~~PO_2~~^- phosphate band region, 1350-1150 cm^- ^1 {{Image200\|LBGPEccp:R:R:YS2ANe005SP0001.gif}} <<2459>>.		\|IR Spectra=Fourier transform IR spectra for dimyristoyl-PE in the dry state (anhydrous), either in the pure state (A, C and E) or in the presence of 20 mol% of alpha -tocopherol (B, D and F). Ester C=O stretching vibration mode region,1800-1700 cm^{-1}; CH^{-1}_2 ; headgroup PO^-_2 phosphate band region, 1350-1150 cm^{-1} {{Image200\|LBGPEccp:R:R:YS2ANe005SP0001.gif}} <<2459>>.
	\|NMR Spectra=^1 H-NMR spectrum of PE. Varian VXR500 spectrometer operating at 500 MHz for protons, using CHCl_3 as the lock signal {{Image200\|LBGPEccp:R:R:YS2ANe005SP0002.gif}} <<2460>>./<BR>^~~3 ^1~~ P NMR spectrum of a PE (left) and a PC (right) fraction from bovine brain. Varian Unity 300 spectrometer operating at121.42 MHz using 1% H_3 PO_4 as external standard {{Image200\|LBGPEccp:R:R:YS2ANe005SP0003.gif}} <<2461>>.,		\|NMR Spectra=^1 H-NMR spectrum of PE. Varian VXR500 spectrometer operating at 500 MHz for protons, using CHCl_3 as the lock signal {{Image200\|LBGPEccp:R:R:YS2ANe005SP0002.gif}} <<2460>>./<BR>^{31}P NMR spectrum of a PE (left) and a PC (right) fraction from bovine brain. Varian Unity 300 spectrometer operating at121.42 MHz using 1% H_3 PO_4 as external standard {{Image200\|LBGPEccp:R:R:YS2ANe005SP0003.gif}} <<2461>>.,
	\|Chromatograms=2 dimensional TLC {{Image200\|LBGPEccp:R:R:YS2ANe005CH0001.gif}} <<2463>>/<BR>HPLC, Hewlett-Packard Model 1050 HPLC system, UV absorbance 205nm, Hewlett-Packard Model 3396 series II integrator, HP Hypersil C18 (200 x 2.1x 5 mu m) column {{Image200\|LBGPEccp:R:R:YS2ANe005CH0002.gif}} <<2464>>/<BR> HPLC for intact PE molecular species. Molecular species of approxymately 50 nmol of PE were separated on two 250 x 4 mm Lichrospher RP-18 endcapped columns in series (Merck, Darmstadt, Germany). Isocratic elution was performed with a solvent consisting of acetonitrile-methanol 3:7 (v/v) containing 5 mu M ethanolamine, or a solvent consisiting of acetonitrile-methanol 2:3 (v/v) containing 2 mu M ethanolamine at a flow rate of 1.25 ml/min. The column effuluent was monitored at 206 nm using LKB 2251 Uvicord (Pharmacia, Upsala, Sweden) and subsequent ELSD was performed using a Varex MKIII obtained from Alltech, (Deerfield, IL) operating at a gas flow rate of 1.9 l/ml and a drift tube temperature of 100°C {{Image200\|LBGPEccp:R:R:YS2ANe005CH0003.gif}} {{Image200\|LBGPEccp:R:R:YS2ANe005FT0001.gif}} <<2462>>.,		\|Chromatograms=2 dimensional TLC {{Image200\|LBGPEccp:R:R:YS2ANe005CH0001.gif}} <<2463>>/<BR>HPLC, Hewlett-Packard Model 1050 HPLC system, UV absorbance 205nm, Hewlett-Packard Model 3396 series II integrator, HP Hypersil C18 (200 x 2.1x 5 mu m) column {{Image200\|LBGPEccp:R:R:YS2ANe005CH0002.gif}} <<2464>>/<BR> HPLC for intact PE molecular species. Molecular species of approxymately 50 nmol of PE were separated on two 250 x 4 mm Lichrospher RP-18 endcapped columns in series (Merck, Darmstadt, Germany). Isocratic elution was performed with a solvent consisting of acetonitrile-methanol 3:7 (v/v) containing 5 mu M ethanolamine, or a solvent consisiting of acetonitrile-methanol 2:3 (v/v) containing 2 mu M ethanolamine at a flow rate of 1.25 ml/min. The column effuluent was monitored at 206 nm using LKB 2251 Uvicord (Pharmacia, Upsala, Sweden) and subsequent ELSD was performed using a Varex MKIII obtained from Alltech, (Deerfield, IL) operating at a gas flow rate of 1.9 l/ml and a drift tube temperature of 100°C {{Image200\|LBGPEccp:R:R:YS2ANe005CH0003.gif}} {{Image200\|LBGPEccp:R:R:YS2ANe005FT0001.gif}} <<2462>>.,
	}}		}}

	{{Lipid/Footer}}		{{Lipid/Footer}}

Editor: LBGPEccp:R:R:YS2ANe005:01 moved to LBGPEccp:R:R:YS2ANe005

2010-02-19T14:00:10Z

LBGPEccp:R:R:YS2ANe005:01 moved to LBGPEccp:R:R:YS2ANe005

← Older revision		Revision as of 23:00, 19 February 2010
Line 6:		Line 6:
	\|Common Name=&&phosphatidylethanolamine&& cephalin&& ethanolaminephosphoglyceride&& (3-sn-phosphatidyl) ethanolamine &&L-a-phosphatidylethanolamine&&		\|Common Name=&&phosphatidylethanolamine&& cephalin&& ethanolaminephosphoglyceride&& (3-sn-phosphatidyl) ethanolamine &&L-a-phosphatidylethanolamine&&
	\|Melting Point=196°C <<2455>>; dimyristoyl-PE, 175-177°C <<2456/2457>>; dipalmitoyl-PE, 172-175°C <<2456/2457>> 210-211°C <<2469>> dipalmitoyl-DL-, 211°C <<2469>>; distearoyl-PE, 172-173.5°C <<2456/2457>>; dioleoyl-PE, 195-200°C <<2469>>; dielaidoyl-PE, 193°C <<2469>>; 1-palmitoyl-2-oleoyl-PE, 194-196°C <<2471>>		\|Melting Point=196°C <<2455>>; dimyristoyl-PE, 175-177°C <<2456/2457>>; dipalmitoyl-PE, 172-175°C <<2456/2457>> 210-211°C <<2469>> dipalmitoyl-DL-, 211°C <<2469>>; distearoyl-PE, 172-173.5°C <<2456/2457>>; dioleoyl-PE, 195-200°C <<2469>>; dielaidoyl-PE, 193°C <<2469>>; 1-palmitoyl-2-oleoyl-PE, 194-196°C <<2471>>
	\|Reflactive=dimyristoyl-PE , [alpha]^26_D +6.7° in CHCl_3 <<2456/2457>>; dipalmitoyl-PE, [alpha]^26_D +6.4° in CHCl_3 <<2456/2457>>, [alpha]^25_D +6.2° in CHCl_3 -MeOH (2:1,v/v) <<2469>>; distearoyl-PE, [alpha]^24_D +6.0° in CHCl_3 -glacial acetic acid (9:1,v/v) <<2456/2457>>; dioleoyl-PE, [alpha]^22_D +6.0° in CHCl_3 <<2469>>; dielaidoyl-PE, [alpha]^22_D +6.1° in CHCl_3 <<2469>>; 1-palmitoyl-2-oleoyl-PE, [alpha]^22_D +6.35° in CHCl_3 <<2469>>		\|Reflactive=dimyristoyl-PE , [ alpha ]^26_D +6.7° in CHCl_3 <<2456/2457>>; dipalmitoyl-PE, [ alpha ]^26_D +6.4° in CHCl_3 <<2456/2457>>, [ alpha ]^25_D +6.2° in CHCl_3 -MeOH (2:1,v/v) <<2469>>; distearoyl-PE, [ alpha ]^24_D +6.0° in CHCl_3 -glacial acetic acid (9:1,v/v) <<2456/2457>>; dioleoyl-PE, [ alpha ]^22_D +6.0° in CHCl_3 <<2469>>; dielaidoyl-PE, [ alpha ]^22_D +6.1° in CHCl_3 <<2469>>; 1-palmitoyl-2-oleoyl-PE, [ alpha ]^22_D +6.35° in CHCl_3 <<2469>>
	\|Solubility=Insoluble in water. Natural PE is soluble in MeOH, EtOH, CHCl_3 , benzene, ether, and petroleum ether, and insoluble in acetone. At 20°C, synthetic PEs are insoluble (<=1mg/100 ml of dry solvent) in acetone, ether, petroleum ether, and ethyl acetate; moderately soluble (20-100 mg/100 ml of dry solvent) in EtOH, pyridine, benzene, and CCl_4 ; readily soluble (> 1g/100 ml of solvent) in CHCl_3 <<2455/2456/2457>>.		\|Solubility=Insoluble in water. Natural PE is soluble in MeOH, EtOH, CHCl_3 , benzene, ether, and petroleum ether, and insoluble in acetone. At 20°C, synthetic PEs are insoluble (<=1mg/100 ml of dry solvent) in acetone, ether, petroleum ether, and ethyl acetate; moderately soluble (20-100 mg/100 ml of dry solvent) in EtOH, pyridine, benzene, and CCl_4 ; readily soluble (> 1g/100 ml of solvent) in CHCl_3 <<2455/2456/2457>>.
	\|Mass Spectra=Negative ion mass spectrum during the elution of 1-stearol-2-arachidonyl-PE in a sample of rat liver PE. A Fisons VG Platform II single quadrupole mass spectrometer, fitted with an electrospray ion source operated at atmospheric pressure, was used for the identification of components eluting from the column. Nitrogen was used as nebulizer gas and as curtain gas. The capillary voltage was set to 3.0 kV and the cone voltage to 100 V {{Image200\|LBGPEccp:R:R:YS2ANe005SP0004.gif}} <<2462>>.,		\|Mass Spectra=Negative ion mass spectrum during the elution of 1-stearol-2-arachidonyl-PE in a sample of rat liver PE. A Fisons VG Platform II single quadrupole mass spectrometer, fitted with an electrospray ion source operated at atmospheric pressure, was used for the identification of components eluting from the column. Nitrogen was used as nebulizer gas and as curtain gas. The capillary voltage was set to 3.0 kV and the cone voltage to 100 V {{Image200\|LBGPEccp:R:R:YS2ANe005SP0004.gif}} <<2462>>.,
	\|UV Spectra=Maximum absorbance at 205 nm <<2458>>.		\|UV Spectra=Maximum absorbance at 205 nm <<2458>>.
	\|IR Spectra=Fourier transform IR spectra for dimyristoyl-PE in the dry state (anhydrous), either in the pure state (A, C and E) or in the presence of 20 mol% of alpha-tocopherol (B, D and F). Ester C=O stretching vibration mode region,1800-1700 cm^- ^1 ; CH_2^- ^1 ; headgroup PO_2^- phosphate band region, 1350-1150 cm^- ^1 {{Image200\|LBGPEccp:R:R:YS2ANe005SP0001.gif}} <<2459>>.		\|IR Spectra=Fourier transform IR spectra for dimyristoyl-PE in the dry state (anhydrous), either in the pure state (A, C and E) or in the presence of 20 mol% of alpha -tocopherol (B, D and F). Ester C=O stretching vibration mode region,1800-1700 cm^- ^1 ; CH_2^- ^1 ; headgroup PO_2^- phosphate band region, 1350-1150 cm^- ^1 {{Image200\|LBGPEccp:R:R:YS2ANe005SP0001.gif}} <<2459>>.
	\|NMR Spectra=^1 H-NMR spectrum of PE. Varian VXR500 spectrometer operating at 500 MHz for protons, using CHCl_3 as the lock signal {{Image200\|LBGPEccp:R:R:YS2ANe005SP0002.gif}} <<2460>>./<BR>^3 ^1 P NMR spectrum of a PE (left) and a PC (right) fraction from bovine brain. Varian Unity 300 spectrometer operating at121.42 MHz using 1% H_3 PO_4 as external standard {{Image200\|LBGPEccp:R:R:YS2ANe005SP0003.gif}} <<2461>>.,		\|NMR Spectra=^1 H-NMR spectrum of PE. Varian VXR500 spectrometer operating at 500 MHz for protons, using CHCl_3 as the lock signal {{Image200\|LBGPEccp:R:R:YS2ANe005SP0002.gif}} <<2460>>./<BR>^3 ^1 P NMR spectrum of a PE (left) and a PC (right) fraction from bovine brain. Varian Unity 300 spectrometer operating at121.42 MHz using 1% H_3 PO_4 as external standard {{Image200\|LBGPEccp:R:R:YS2ANe005SP0003.gif}} <<2461>>.,
	\|Chromatograms=2 dimensional TLC {{Image200\|LBGPEccp:R:R:YS2ANe005CH0001.gif}} <<2463>>/<BR>HPLC, Hewlett-Packard Model 1050 HPLC system, UV absorbance 205nm, Hewlett-Packard Model 3396 series II integrator, HP Hypersil C18 (200 x 2.1x 5 ~~mum~~) column {{Image200\|LBGPEccp:R:R:YS2ANe005CH0002.gif}} <<2464>>/<BR> HPLC for intact PE molecular species. Molecular species of approxymately 50 nmol of PE were separated on two 250 x 4 mm Lichrospher RP-18 endcapped columns in series (Merck, Darmstadt, Germany). Isocratic elution was performed with a solvent consisting of acetonitrile-methanol 3:7 (v/v) containing 5 ~~muM~~ ethanolamine, or a solvent consisiting of acetonitrile-methanol 2:3 (v/v) containing 2 ~~muM~~ ethanolamine at a flow rate of 1.25 ml/min. The column effuluent was monitored at 206 nm using LKB 2251 Uvicord (Pharmacia, Upsala, Sweden) and subsequent ELSD was performed using a Varex MKIII obtained from Alltech, (Deerfield, IL) operating at a gas flow rate of 1.9 l/ml and a drift tube temperature of 100°C {{Image200\|LBGPEccp:R:R:YS2ANe005CH0003.gif}} {{Image200\|LBGPEccp:R:R:YS2ANe005FT0001.gif}} <<2462>>.,		\|Chromatograms=2 dimensional TLC {{Image200\|LBGPEccp:R:R:YS2ANe005CH0001.gif}} <<2463>>/<BR>HPLC, Hewlett-Packard Model 1050 HPLC system, UV absorbance 205nm, Hewlett-Packard Model 3396 series II integrator, HP Hypersil C18 (200 x 2.1x 5 mu m) column {{Image200\|LBGPEccp:R:R:YS2ANe005CH0002.gif}} <<2464>>/<BR> HPLC for intact PE molecular species. Molecular species of approxymately 50 nmol of PE were separated on two 250 x 4 mm Lichrospher RP-18 endcapped columns in series (Merck, Darmstadt, Germany). Isocratic elution was performed with a solvent consisting of acetonitrile-methanol 3:7 (v/v) containing 5 mu M ethanolamine, or a solvent consisiting of acetonitrile-methanol 2:3 (v/v) containing 2 mu M ethanolamine at a flow rate of 1.25 ml/min. The column effuluent was monitored at 206 nm using LKB 2251 Uvicord (Pharmacia, Upsala, Sweden) and subsequent ELSD was performed using a Varex MKIII obtained from Alltech, (Deerfield, IL) operating at a gas flow rate of 1.9 l/ml and a drift tube temperature of 100°C {{Image200\|LBGPEccp:R:R:YS2ANe005CH0003.gif}} {{Image200\|LBGPEccp:R:R:YS2ANe005FT0001.gif}} <<2462>>.,
	}}		}}

	{{Lipid/Footer}}		{{Lipid/Footer}}

Editor: LBGPEccp:R:R:YS2ANe005:01 moved to LBGPEccp:R:R:YS2ANe005

2010-02-19T14:00:00Z

LBGPEccp:R:R:YS2ANe005:01 moved to LBGPEccp:R:R:YS2ANe005

← Older revision		Revision as of 23:00, 19 February 2010
Line 6:		Line 6:
	\|Common Name=&&phosphatidylethanolamine&& cephalin&& ethanolaminephosphoglyceride&& (3-sn-phosphatidyl) ethanolamine &&L-a-phosphatidylethanolamine&&		\|Common Name=&&phosphatidylethanolamine&& cephalin&& ethanolaminephosphoglyceride&& (3-sn-phosphatidyl) ethanolamine &&L-a-phosphatidylethanolamine&&
	\|Melting Point=196°C <<2455>>; dimyristoyl-PE, 175-177°C <<2456/2457>>; dipalmitoyl-PE, 172-175°C <<2456/2457>> 210-211°C <<2469>> dipalmitoyl-DL-, 211°C <<2469>>; distearoyl-PE, 172-173.5°C <<2456/2457>>; dioleoyl-PE, 195-200°C <<2469>>; dielaidoyl-PE, 193°C <<2469>>; 1-palmitoyl-2-oleoyl-PE, 194-196°C <<2471>>		\|Melting Point=196°C <<2455>>; dimyristoyl-PE, 175-177°C <<2456/2457>>; dipalmitoyl-PE, 172-175°C <<2456/2457>> 210-211°C <<2469>> dipalmitoyl-DL-, 211°C <<2469>>; distearoyl-PE, 172-173.5°C <<2456/2457>>; dioleoyl-PE, 195-200°C <<2469>>; dielaidoyl-PE, 193°C <<2469>>; 1-palmitoyl-2-oleoyl-PE, 194-196°C <<2471>>
	\|Reflactive=dimyristoyl-PE , [~~<FONT FACE="Symbol">a</FONT>~~]~~<sub>D</sub><sup>26</sup>~~+6.7° in ~~CHCl<SUB><FONT SIZE=-1>3</FONT></SUB>~~ <<2456/2457>>; dipalmitoyl-PE, [~~<FONT FACE="Symbol">a</FONT>~~]~~<sub>D</sub><sup>26</sup>~~+6.4° in ~~CHCl<SUB><FONT SIZE=-1>3</FONT></SUB>~~ <<2456/2457>>, [~~<FONT FACE="Symbol">a</FONT>~~]~~<sub>D</sub><sup>25</sup>~~+6.2° in ~~CHCl<SUB><FONT SIZE=-1>3</FONT></SUB>~~-MeOH (2:1,v/v) <<2469>>; distearoyl-PE, [~~<FONT FACE="Symbol">a</FONT>~~]~~<sub>D</sub><sup>24</sup>~~+6.0° in ~~CHCl<SUB><FONT SIZE=-1>3</FONT></SUB>~~-glacial acetic acid (9:1,v/v) <<2456/2457>>; dioleoyl-PE, [~~<FONT FACE="Symbol">a</FONT>~~]~~<sub>D</sub><sup>22</sup>~~+6.0° in ~~CHCl<SUB><FONT SIZE=-1>3</FONT></SUB>~~ <<2469>>; dielaidoyl-PE, [~~<FONT FACE="Symbol">a</FONT>~~]~~<sub>D</sub><sup>22</sup>~~+6.1° in ~~CHCl<SUB><FONT SIZE=-1>3</FONT></SUB>~~ <<2469>>; 1-palmitoyl-2-oleoyl-PE, [~~<FONT FACE="Symbol">a</FONT>~~]~~<sub>D</sub><sup>22</sup>~~+6.35° in ~~CHCl<SUB><FONT SIZE=-1>3</FONT></SUB>~~ <<2469>>		\|Reflactive=dimyristoyl-PE , [alpha]^26_D +6.7° in CHCl_3 <<2456/2457>>; dipalmitoyl-PE, [alpha]^26_D +6.4° in CHCl_3 <<2456/2457>>, [alpha]^25_D +6.2° in CHCl_3 -MeOH (2:1,v/v) <<2469>>; distearoyl-PE, [alpha]^24_D +6.0° in CHCl_3 -glacial acetic acid (9:1,v/v) <<2456/2457>>; dioleoyl-PE, [alpha]^22_D +6.0° in CHCl_3 <<2469>>; dielaidoyl-PE, [alpha]^22_D +6.1° in CHCl_3 <<2469>>; 1-palmitoyl-2-oleoyl-PE, [alpha]^22_D +6.35° in CHCl_3 <<2469>>
	\|Solubility=Insoluble in water. Natural PE is soluble in MeOH, EtOH, ~~CHCl<SUB><FONT SIZE=-1>3</FONT></SUB>~~, benzene, ether, and petroleum ether, and insoluble in acetone. At 20°C, synthetic PEs are insoluble (<=1mg/100 ml of dry solvent) in acetone, ether, petroleum ether, and ethyl acetate; moderately soluble (20-100 mg/100 ml of dry solvent) in EtOH, pyridine, benzene, and ~~CCl<SUB><FONT SIZE=-1>4</FONT></SUB>~~; readily soluble (> 1g/100 ml of solvent) in ~~CHCl<SUB><FONT SIZE=-1>3</FONT></SUB>~~ <<2455/2456/2457>>.		\|Solubility=Insoluble in water. Natural PE is soluble in MeOH, EtOH, CHCl_3 , benzene, ether, and petroleum ether, and insoluble in acetone. At 20°C, synthetic PEs are insoluble (<=1mg/100 ml of dry solvent) in acetone, ether, petroleum ether, and ethyl acetate; moderately soluble (20-100 mg/100 ml of dry solvent) in EtOH, pyridine, benzene, and CCl_4 ; readily soluble (> 1g/100 ml of solvent) in CHCl_3 <<2455/2456/2457>>.
	\|Mass Spectra=Negative ion mass spectrum during the elution of 1-stearol-2-arachidonyl-PE in a sample of rat liver PE. A Fisons VG Platform II single quadrupole mass spectrometer, fitted with an electrospray ion source operated at atmospheric pressure, was used for the identification of components eluting from the column. Nitrogen was used as nebulizer gas and as curtain gas. The capillary voltage was set to 3.0 kV and the cone voltage to 100 V {{Image200\|LBGPEccp:R:R:YS2ANe005SP0004.gif}} <<2462>>.,		\|Mass Spectra=Negative ion mass spectrum during the elution of 1-stearol-2-arachidonyl-PE in a sample of rat liver PE. A Fisons VG Platform II single quadrupole mass spectrometer, fitted with an electrospray ion source operated at atmospheric pressure, was used for the identification of components eluting from the column. Nitrogen was used as nebulizer gas and as curtain gas. The capillary voltage was set to 3.0 kV and the cone voltage to 100 V {{Image200\|LBGPEccp:R:R:YS2ANe005SP0004.gif}} <<2462>>.,
	\|UV Spectra=Maximum absorbance at 205 nm <<2458>>.		\|UV Spectra=Maximum absorbance at 205 nm <<2458>>.
	\|IR Spectra=Fourier transform IR spectra for dimyristoyl-PE in the dry state (anhydrous), either in the pure state (A, C and E) or in the presence of 20 mol% of ~~<FONT FACE="Symbol">a</FONT>~~-tocopherol (B, D and F). Ester C=O stretching vibration mode region,1800-1700 cm~~<SUP><FONT SIZE=~~-1~~>-</FONT></SUP><SUP><FONT SIZE=-1>1</FONT></SUP>~~; ~~CH<SUB><FONT SIZE=-1>2</FONT></SUB> deformation, scissoring band region, 1500-1400 cm<SUP><FONT SIZE=-1>-</FONT></SUP><SUP><FONT SIZE=~~-1~~>1</FONT></SUP>~~; headgroup ~~PO<SUB><FONT SIZE=-1>2</FONT></SUB><SUP><FONT SIZE=~~-~~1>-</FONT></SUP>~~ phosphate band region, 1350-1150 cm~~<SUP><FONT SIZE=-1>-</FONT></SUP><SUP><FONT SIZE=~~-1~~>1</FONT></SUP>~~ {{Image200\|LBGPEccp:R:R:YS2ANe005SP0001.gif}} <<2459>>.		\|IR Spectra=Fourier transform IR spectra for dimyristoyl-PE in the dry state (anhydrous), either in the pure state (A, C and E) or in the presence of 20 mol% of alpha-tocopherol (B, D and F). Ester C=O stretching vibration mode region,1800-1700 cm^- ^1 ; CH_2^- ^1 ; headgroup PO_2^- phosphate band region, 1350-1150 cm^- ^1 {{Image200\|LBGPEccp:R:R:YS2ANe005SP0001.gif}} <<2459>>.
	\|NMR Spectra=~~<SUP><FONT SIZE=-1>~~1~~</FONT></SUP>~~H-NMR spectrum of PE. Varian VXR500 spectrometer operating at 500 MHz for protons, using ~~CHCl<SUB><FONT SIZE=-1>3</FONT></SUB>~~ as the lock signal {{Image200\|LBGPEccp:R:R:YS2ANe005SP0002.gif}} <<2460>>./<BR>~~<SUP><FONT SIZE=-1>~~3~~</FONT></SUP><SUP><FONT SIZE=-1>~~1~~</FONT></SUP>~~P NMR spectrum of a PE (left) and a PC (right) fraction from bovine brain. Varian Unity 300 spectrometer operating at121.42 MHz using 1% ~~H<SUB><FONT SIZE=-1>3</FONT></SUB>PO<SUB><FONT SIZE=-1>4</FONT></SUB>~~ as external standard {{Image200\|LBGPEccp:R:R:YS2ANe005SP0003.gif}} <<2461>>.,		\|NMR Spectra=^1 H-NMR spectrum of PE. Varian VXR500 spectrometer operating at 500 MHz for protons, using CHCl_3 as the lock signal {{Image200\|LBGPEccp:R:R:YS2ANe005SP0002.gif}} <<2460>>./<BR>^3 ^1 P NMR spectrum of a PE (left) and a PC (right) fraction from bovine brain. Varian Unity 300 spectrometer operating at121.42 MHz using 1% H_3 PO_4 as external standard {{Image200\|LBGPEccp:R:R:YS2ANe005SP0003.gif}} <<2461>>.,
	\|Chromatograms=2 dimensional TLC {{Image200\|LBGPEccp:R:R:YS2ANe005CH0001.gif}} <<2463>>/<BR>HPLC, Hewlett-Packard Model 1050 HPLC system, UV absorbance 205nm, Hewlett-Packard Model 3396 series II integrator, HP Hypersil C18 (200 x 2.1x 5 ~~<FONT FACE="Symbol">m</FONT>m~~) column {{Image200\|LBGPEccp:R:R:YS2ANe005CH0002.gif}} <<2464>>/<BR> HPLC for intact PE molecular species. Molecular species of approxymately 50 nmol of PE were separated on two 250 x 4 mm Lichrospher RP-18 endcapped columns in series (Merck, Darmstadt, Germany). Isocratic elution was performed with a solvent consisting of acetonitrile-methanol 3:7 (v/v) containing 5 ~~<FONT FACE="Symbol">m</FONT>M~~ ethanolamine, or a solvent consisiting of acetonitrile-methanol 2:3 (v/v) containing 2 ~~<FONT FACE="Symbol">m</FONT>M~~ ethanolamine at a flow rate of 1.25 ml/min. The column effuluent was monitored at 206 nm using LKB 2251 Uvicord (Pharmacia, Upsala, Sweden) and subsequent ELSD was performed using a Varex MKIII obtained from Alltech, (Deerfield, IL) operating at a gas flow rate of 1.9 l/ml and a drift tube temperature of 100°C {{Image200\|LBGPEccp:R:R:YS2ANe005CH0003.gif}} {{Image200\|LBGPEccp:R:R:YS2ANe005FT0001.gif}} <<2462>>.,		\|Chromatograms=2 dimensional TLC {{Image200\|LBGPEccp:R:R:YS2ANe005CH0001.gif}} <<2463>>/<BR>HPLC, Hewlett-Packard Model 1050 HPLC system, UV absorbance 205nm, Hewlett-Packard Model 3396 series II integrator, HP Hypersil C18 (200 x 2.1x 5 mum) column {{Image200\|LBGPEccp:R:R:YS2ANe005CH0002.gif}} <<2464>>/<BR> HPLC for intact PE molecular species. Molecular species of approxymately 50 nmol of PE were separated on two 250 x 4 mm Lichrospher RP-18 endcapped columns in series (Merck, Darmstadt, Germany). Isocratic elution was performed with a solvent consisting of acetonitrile-methanol 3:7 (v/v) containing 5 muM ethanolamine, or a solvent consisiting of acetonitrile-methanol 2:3 (v/v) containing 2 muM ethanolamine at a flow rate of 1.25 ml/min. The column effuluent was monitored at 206 nm using LKB 2251 Uvicord (Pharmacia, Upsala, Sweden) and subsequent ELSD was performed using a Varex MKIII obtained from Alltech, (Deerfield, IL) operating at a gas flow rate of 1.9 l/ml and a drift tube temperature of 100°C {{Image200\|LBGPEccp:R:R:YS2ANe005CH0003.gif}} {{Image200\|LBGPEccp:R:R:YS2ANe005FT0001.gif}} <<2462>>.,
	}}		}}

	{{Lipid/Footer}}		{{Lipid/Footer}}

Editor: LBGPEccp:R:R:YS2ANe005:01 moved to LBGPEccp:R:R:YS2ANe005

2010-02-18T15:00:00Z

LBGPEccp:R:R:YS2ANe005:01 moved to LBGPEccp:R:R:YS2ANe005

← Older revision		Revision as of 00:00, 19 February 2010
Line 4:		Line 4:
	\|LipidBank=PGP2410		\|LipidBank=PGP2410
	\|SysName=L-a-phosphatidylethanolamine		\|SysName=L-a-phosphatidylethanolamine
	\|Common Name=phosphatidylethanolamine cephalin ethanolaminephosphoglyceride (3-sn-phosphatidyl) ethanolamine L-a-phosphatidylethanolamine		\|Common Name=&&phosphatidylethanolamine&& cephalin&& ethanolaminephosphoglyceride&& (3-sn-phosphatidyl) ethanolamine &&L-a-phosphatidylethanolamine&&
	\|Melting Point=196°C 2455; dimyristoyl-PE, 175-177°C 2456/2457; dipalmitoyl-PE, 172-175°C 2456/2457 210-211°C 2469 dipalmitoyl-DL-, 211°C 2469; distearoyl-PE, 172-173.5°C 2456/2457; dioleoyl-PE, 195-200°C 2469; dielaidoyl-PE, 193°C 2469; 1-palmitoyl-2-oleoyl-PE, 194-196°C 2471		\|Melting Point=196°C <<2455>>; dimyristoyl-PE, 175-177°C <<2456/2457>>; dipalmitoyl-PE, 172-175°C <<2456/2457>> 210-211°C <<2469>> dipalmitoyl-DL-, 211°C <<2469>>; distearoyl-PE, 172-173.5°C <<2456/2457>>; dioleoyl-PE, 195-200°C <<2469>>; dielaidoyl-PE, 193°C <<2469>>; 1-palmitoyl-2-oleoyl-PE, 194-196°C <<2471>>
	\|Reflactive=dimyristoyl-PE , [FONT FACE=~~Symbola~~/FONT]~~subD~~/~~subsup26~~/sup+6.7° in ~~CHClSUBFONT~~ SIZE=-13/FONT/SUB 2456/2457; dipalmitoyl-PE, [FONT FACE=~~Symbola~~/FONT]~~subD~~/~~subsup26~~/sup+6.4° in ~~CHClSUBFONT~~ SIZE=-13/FONT/SUB 2456/2457, [FONT FACE=~~Symbola~~/FONT]~~subD~~/~~subsup25~~/sup+6.2° in ~~CHClSUBFONT~~ SIZE=-13/FONT/SUB-MeOH (2:1,v/v) 2469; distearoyl-PE, [FONT FACE=~~Symbola~~/FONT]~~subD~~/~~subsup24~~/sup+6.0° in ~~CHClSUBFONT~~ SIZE=-13/FONT/SUB-glacial acetic acid (9:1,v/v) 2456/2457; dioleoyl-PE, [FONT FACE=~~Symbola~~/FONT]~~subD~~/~~subsup22~~/sup+6.0° in ~~CHClSUBFONT~~ SIZE=-13/FONT/SUB 2469; dielaidoyl-PE, [FONT FACE=~~Symbola~~/FONT]~~subD~~/~~subsup22~~/sup+6.1° in ~~CHClSUBFONT~~ SIZE=-13/FONT/SUB 2469; 1-palmitoyl-2-oleoyl-PE, [FONT FACE=~~Symbola~~/FONT]~~subD~~/~~subsup22~~/sup+6.35° in ~~CHClSUBFONT~~ SIZE=-13/FONT/SUB 2469		\|Reflactive=dimyristoyl-PE , [<FONT FACE="Symbol">a</FONT>]<sub>D</sub><sup>26</sup>+6.7° in CHCl<SUB><FONT SIZE=-1>3</FONT></SUB> <<2456/2457>>; dipalmitoyl-PE, [<FONT FACE="Symbol">a</FONT>]<sub>D</sub><sup>26</sup>+6.4° in CHCl<SUB><FONT SIZE=-1>3</FONT></SUB> <<2456/2457>>, [<FONT FACE="Symbol">a</FONT>]<sub>D</sub><sup>25</sup>+6.2° in CHCl<SUB><FONT SIZE=-1>3</FONT></SUB>-MeOH (2:1,v/v) <<2469>>; distearoyl-PE, [<FONT FACE="Symbol">a</FONT>]<sub>D</sub><sup>24</sup>+6.0° in CHCl<SUB><FONT SIZE=-1>3</FONT></SUB>-glacial acetic acid (9:1,v/v) <<2456/2457>>; dioleoyl-PE, [<FONT FACE="Symbol">a</FONT>]<sub>D</sub><sup>22</sup>+6.0° in CHCl<SUB><FONT SIZE=-1>3</FONT></SUB> <<2469>>; dielaidoyl-PE, [<FONT FACE="Symbol">a</FONT>]<sub>D</sub><sup>22</sup>+6.1° in CHCl<SUB><FONT SIZE=-1>3</FONT></SUB> <<2469>>; 1-palmitoyl-2-oleoyl-PE, [<FONT FACE="Symbol">a</FONT>]<sub>D</sub><sup>22</sup>+6.35° in CHCl<SUB><FONT SIZE=-1>3</FONT></SUB> <<2469>>
	\|Solubility=Insoluble in water. Natural PE is soluble in MeOH, EtOH, ~~CHClSUBFONT~~ SIZE=-13/FONT/SUB, benzene, ether, and petroleum ether, and insoluble in acetone. At 20°C, synthetic PEs are insoluble (=1mg/100 ml of dry solvent) in acetone, ether, petroleum ether, and ethyl acetate; moderately soluble (20-100 mg/100 ml of dry solvent) in EtOH, pyridine, benzene, and ~~CClSUBFONT~~ SIZE=-14/FONT/SUB; readily soluble ( 1g/100 ml of solvent) in ~~CHClSUBFONT~~ SIZE=-13/FONT/SUB 2455/2456/2457.		\|Solubility=Insoluble in water. Natural PE is soluble in MeOH, EtOH, CHCl<SUB><FONT SIZE=-1>3</FONT></SUB>, benzene, ether, and petroleum ether, and insoluble in acetone. At 20°C, synthetic PEs are insoluble (<=1mg/100 ml of dry solvent) in acetone, ether, petroleum ether, and ethyl acetate; moderately soluble (20-100 mg/100 ml of dry solvent) in EtOH, pyridine, benzene, and CCl<SUB><FONT SIZE=-1>4</FONT></SUB>; readily soluble (> 1g/100 ml of solvent) in CHCl<SUB><FONT SIZE=-1>3</FONT></SUB> <<2455/2456/2457>>.
	\|Mass Spectra=Negative ion mass spectrum during the elution of 1-stearol-2-arachidonyl-PE in a sample of rat liver PE. A Fisons VG Platform II single quadrupole mass spectrometer, fitted with an electrospray ion source operated at atmospheric pressure, was used for the identification of components eluting from the column. Nitrogen was used as nebulizer gas and as curtain gas. The capillary voltage was set to 3.0 kV and the cone voltage to 100 V {{Image200\|LBGPEccp:R:R:YS2ANe005SP0004.gif}} 2462.,		\|Mass Spectra=Negative ion mass spectrum during the elution of 1-stearol-2-arachidonyl-PE in a sample of rat liver PE. A Fisons VG Platform II single quadrupole mass spectrometer, fitted with an electrospray ion source operated at atmospheric pressure, was used for the identification of components eluting from the column. Nitrogen was used as nebulizer gas and as curtain gas. The capillary voltage was set to 3.0 kV and the cone voltage to 100 V {{Image200\|LBGPEccp:R:R:YS2ANe005SP0004.gif}} <<2462>>.,
	\|UV Spectra=Maximum absorbance at 205 nm 2458.		\|UV Spectra=Maximum absorbance at 205 nm <<2458>>.
	\|IR Spectra=Fourier transform IR spectra for dimyristoyl-PE in the dry state (anhydrous), either in the pure state (A, C and E) or in the presence of 20 mol% of FONT FACE=~~Symbola~~/FONT-tocopherol (B, D and F). Ester C=O stretching vibration mode region,1800-1700 ~~cmSUPFONT~~ SIZE=-1-/FONT/~~SUPSUPFONT~~ SIZE=-11/FONT/SUP; ~~CHSUBFONT~~ SIZE=-12/FONT/SUB deformation, scissoring band region, 1500-1400 ~~cmSUPFONT~~ SIZE=-1-/FONT/~~SUPSUPFONT~~ SIZE=-11/FONT/SUP; headgroup ~~POSUBFONT~~ SIZE=-12/FONT/~~SUBSUPFONT~~ SIZE=-1-/FONT/SUP phosphate band region, 1350-1150 ~~cmSUPFONT~~ SIZE=-1-/FONT/~~SUPSUPFONT~~ SIZE=-11/FONT/SUP {{Image200\|LBGPEccp:R:R:YS2ANe005SP0001.gif}} 2459.		\|IR Spectra=Fourier transform IR spectra for dimyristoyl-PE in the dry state (anhydrous), either in the pure state (A, C and E) or in the presence of 20 mol% of <FONT FACE="Symbol">a</FONT>-tocopherol (B, D and F). Ester C=O stretching vibration mode region,1800-1700 cm<SUP><FONT SIZE=-1>-</FONT></SUP><SUP><FONT SIZE=-1>1</FONT></SUP>; CH<SUB><FONT SIZE=-1>2</FONT></SUB> deformation, scissoring band region, 1500-1400 cm<SUP><FONT SIZE=-1>-</FONT></SUP><SUP><FONT SIZE=-1>1</FONT></SUP>; headgroup PO<SUB><FONT SIZE=-1>2</FONT></SUB><SUP><FONT SIZE=-1>-</FONT></SUP> phosphate band region, 1350-1150 cm<SUP><FONT SIZE=-1>-</FONT></SUP><SUP><FONT SIZE=-1>1</FONT></SUP> {{Image200\|LBGPEccp:R:R:YS2ANe005SP0001.gif}} <<2459>>.
	\|NMR Spectra=~~SUPFONT~~ SIZE=-11/FONT/~~SUPH~~-NMR spectrum of PE. Varian VXR500 spectrometer operating at 500 MHz for protons, using ~~CHClSUBFONT~~ SIZE=-13/FONT/SUB as the lock signal {{Image200\|LBGPEccp:R:R:YS2ANe005SP0002.gif}} 2460./~~BRSUPFONT~~ SIZE=-13/FONT/~~SUPSUPFONT~~ SIZE=-11/FONT/~~SUPP~~ NMR spectrum of a PE (left) and a PC (right) fraction from bovine brain. Varian Unity 300 spectrometer operating at121.42 MHz using 1% ~~HSUBFONT~~ SIZE=-13/FONT/~~SUBPOSUBFONT~~ SIZE=-14/FONT/SUB as external standard {{Image200\|LBGPEccp:R:R:YS2ANe005SP0003.gif}} 2461.,		\|NMR Spectra=<SUP><FONT SIZE=-1>1</FONT></SUP>H-NMR spectrum of PE. Varian VXR500 spectrometer operating at 500 MHz for protons, using CHCl<SUB><FONT SIZE=-1>3</FONT></SUB> as the lock signal {{Image200\|LBGPEccp:R:R:YS2ANe005SP0002.gif}} <<2460>>./<BR><SUP><FONT SIZE=-1>3</FONT></SUP><SUP><FONT SIZE=-1>1</FONT></SUP>P NMR spectrum of a PE (left) and a PC (right) fraction from bovine brain. Varian Unity 300 spectrometer operating at121.42 MHz using 1% H<SUB><FONT SIZE=-1>3</FONT></SUB>PO<SUB><FONT SIZE=-1>4</FONT></SUB> as external standard {{Image200\|LBGPEccp:R:R:YS2ANe005SP0003.gif}} <<2461>>.,
	\|Chromatograms=2 dimensional TLC {{Image200\|LBGPEccp:R:R:YS2ANe005CH0001.gif}} 2463/~~BRHPLC~~, Hewlett-Packard Model 1050 HPLC system, UV absorbance 205nm, Hewlett-Packard Model 3396 series II integrator, HP Hypersil C18 (200 x 2.1x 5 FONT FACE=~~Symbolm~~/~~FONTm~~) column {{Image200\|LBGPEccp:R:R:YS2ANe005CH0002.gif}} 2464/BR HPLC for intact PE molecular species. Molecular species of approxymately 50 nmol of PE were separated on two 250 x 4 mm Lichrospher RP-18 endcapped columns in series (Merck, Darmstadt, Germany). Isocratic elution was performed with a solvent consisting of acetonitrile-methanol 3:7 (v/v) containing 5 FONT FACE=~~Symbolm~~/~~FONTM~~ ethanolamine, or a solvent consisiting of acetonitrile-methanol 2:3 (v/v) containing 2 FONT FACE=~~Symbolm~~/~~FONTM~~ ethanolamine at a flow rate of 1.25 ml/min. The column effuluent was monitored at 206 nm using LKB 2251 Uvicord (Pharmacia, Upsala, Sweden) and subsequent ELSD was performed using a Varex MKIII obtained from Alltech, (Deerfield, IL) operating at a gas flow rate of 1.9 l/ml and a drift tube temperature of 100°C {{Image200\|LBGPEccp:R:R:YS2ANe005CH0003.gif}} {{Image200\|LBGPEccp:R:R:YS2ANe005FT0001.gif}} 2462.,		\|Chromatograms=2 dimensional TLC {{Image200\|LBGPEccp:R:R:YS2ANe005CH0001.gif}} <<2463>>/<BR>HPLC, Hewlett-Packard Model 1050 HPLC system, UV absorbance 205nm, Hewlett-Packard Model 3396 series II integrator, HP Hypersil C18 (200 x 2.1x 5 <FONT FACE="Symbol">m</FONT>m) column {{Image200\|LBGPEccp:R:R:YS2ANe005CH0002.gif}} <<2464>>/<BR> HPLC for intact PE molecular species. Molecular species of approxymately 50 nmol of PE were separated on two 250 x 4 mm Lichrospher RP-18 endcapped columns in series (Merck, Darmstadt, Germany). Isocratic elution was performed with a solvent consisting of acetonitrile-methanol 3:7 (v/v) containing 5 <FONT FACE="Symbol">m</FONT>M ethanolamine, or a solvent consisiting of acetonitrile-methanol 2:3 (v/v) containing 2 <FONT FACE="Symbol">m</FONT>M ethanolamine at a flow rate of 1.25 ml/min. The column effuluent was monitored at 206 nm using LKB 2251 Uvicord (Pharmacia, Upsala, Sweden) and subsequent ELSD was performed using a Varex MKIII obtained from Alltech, (Deerfield, IL) operating at a gas flow rate of 1.9 l/ml and a drift tube temperature of 100°C {{Image200\|LBGPEccp:R:R:YS2ANe005CH0003.gif}} {{Image200\|LBGPEccp:R:R:YS2ANe005FT0001.gif}} <<2462>>.,
	}}		}}

	{{Lipid/Footer}}		{{Lipid/Footer}}

Editor: LBGPEccp:R:R:YS2ANe005:01 moved to LBGPEccp:R:R:YS2ANe005

2010-01-27T20:00:00Z

LBGPEccp:R:R:YS2ANe005:01 moved to LBGPEccp:R:R:YS2ANe005

← Older revision		Revision as of 05:00, 28 January 2010
Line 4:		Line 4:
	\|LipidBank=PGP2410		\|LipidBank=PGP2410
	\|SysName=L-a-phosphatidylethanolamine		\|SysName=L-a-phosphatidylethanolamine
	\|Common Name=&&phosphatidylethanolamine&& cephalin&& ethanolaminephosphoglyceride&& (3-sn-phosphatidyl) ethanolamine &&L-a-phosphatidylethanolamine&&		\|Common Name=phosphatidylethanolamine cephalin ethanolaminephosphoglyceride (3-sn-phosphatidyl) ethanolamine L-a-phosphatidylethanolamine
	\|Melting Point=196°C <<2455>>; dimyristoyl-PE, 175-177°C <<2456/2457>>; dipalmitoyl-PE, 172-175°C <<2456/2457>> 210-211°C <<2469>> dipalmitoyl-DL-, 211°C <<2469>>; distearoyl-PE, 172-173.5°C <<2456/2457>>; dioleoyl-PE, 195-200°C <<2469>>; dielaidoyl-PE, 193°C <<2469>>; 1-palmitoyl-2-oleoyl-PE, 194-196°C <<2471>>		\|Melting Point=196°C 2455; dimyristoyl-PE, 175-177°C 2456/2457; dipalmitoyl-PE, 172-175°C 2456/2457 210-211°C 2469 dipalmitoyl-DL-, 211°C 2469; distearoyl-PE, 172-173.5°C 2456/2457; dioleoyl-PE, 195-200°C 2469; dielaidoyl-PE, 193°C 2469; 1-palmitoyl-2-oleoyl-PE, 194-196°C 2471
	\|Reflactive=dimyristoyl-PE , [<FONT FACE=~~"Symbol">a<~~/FONT>]~~<sub>D<~~/~~sub><sup>26<~~/sup>+6.7° in ~~CHCl<SUB><FONT~~ SIZE=-~~1>3<~~/FONT></SUB~~> <<~~2456/2457>>; dipalmitoyl-PE, [<FONT FACE=~~"Symbol">a<~~/FONT>]~~<sub>D<~~/~~sub><sup>26<~~/sup>+6.4° in ~~CHCl<SUB><FONT~~ SIZE=-~~1>3<~~/FONT></SUB~~> <<~~2456/2457>>, [<FONT FACE=~~"Symbol">a<~~/FONT>]~~<sub>D<~~/~~sub><sup>25<~~/sup>+6.2° in ~~CHCl<SUB><FONT~~ SIZE=-~~1>3<~~/FONT></SUB>-MeOH (2:1,v/v) <<2469>>; distearoyl-PE, [<FONT FACE=~~"Symbol">a<~~/FONT>]~~<sub>D<~~/~~sub><sup>24<~~/sup>+6.0° in ~~CHCl<SUB><FONT~~ SIZE=-~~1>3<~~/FONT></SUB>-glacial acetic acid (9:1,v/v) <<2456/2457>>; dioleoyl-PE, [<FONT FACE=~~"Symbol">a<~~/FONT>]~~<sub>D<~~/~~sub><sup>22<~~/sup>+6.0° in ~~CHCl<SUB><FONT~~ SIZE=-~~1>3<~~/FONT></SUB~~> <<~~2469>>; dielaidoyl-PE, [<FONT FACE=~~"Symbol">a<~~/FONT>]~~<sub>D<~~/~~sub><sup>22<~~/sup>+6.1° in ~~CHCl<SUB><FONT~~ SIZE=-~~1>3<~~/FONT></SUB~~> <<~~2469>>; 1-palmitoyl-2-oleoyl-PE, [<FONT FACE=~~"Symbol">a<~~/FONT>]~~<sub>D<~~/~~sub><sup>22<~~/sup>+6.35° in ~~CHCl<SUB><FONT~~ SIZE=-~~1>3<~~/FONT></SUB~~> <<~~2469>>		\|Reflactive=dimyristoyl-PE , [FONT FACE=Symbola/FONT]subD/subsup26/sup+6.7° in CHClSUBFONT SIZE=-13/FONT/SUB 2456/2457; dipalmitoyl-PE, [FONT FACE=Symbola/FONT]subD/subsup26/sup+6.4° in CHClSUBFONT SIZE=-13/FONT/SUB 2456/2457, [FONT FACE=Symbola/FONT]subD/subsup25/sup+6.2° in CHClSUBFONT SIZE=-13/FONT/SUB-MeOH (2:1,v/v) 2469; distearoyl-PE, [FONT FACE=Symbola/FONT]subD/subsup24/sup+6.0° in CHClSUBFONT SIZE=-13/FONT/SUB-glacial acetic acid (9:1,v/v) 2456/2457; dioleoyl-PE, [FONT FACE=Symbola/FONT]subD/subsup22/sup+6.0° in CHClSUBFONT SIZE=-13/FONT/SUB 2469; dielaidoyl-PE, [FONT FACE=Symbola/FONT]subD/subsup22/sup+6.1° in CHClSUBFONT SIZE=-13/FONT/SUB 2469; 1-palmitoyl-2-oleoyl-PE, [FONT FACE=Symbola/FONT]subD/subsup22/sup+6.35° in CHClSUBFONT SIZE=-13/FONT/SUB 2469
	\|Solubility=Insoluble in water. Natural PE is soluble in MeOH, EtOH, ~~CHCl<SUB><FONT~~ SIZE=-~~1>3<~~/FONT></SUB>, benzene, ether, and petroleum ether, and insoluble in acetone. At 20°C, synthetic PEs are insoluble (<=1mg/100 ml of dry solvent) in acetone, ether, petroleum ether, and ethyl acetate; moderately soluble (20-100 mg/100 ml of dry solvent) in EtOH, pyridine, benzene, and ~~CCl<SUB><FONT~~ SIZE=-~~1>4<~~/FONT></SUB>; readily soluble (> 1g/100 ml of solvent) in ~~CHCl<SUB><FONT~~ SIZE=-~~1>3<~~/FONT></SUB~~> <<~~2455/2456/2457>>.		\|Solubility=Insoluble in water. Natural PE is soluble in MeOH, EtOH, CHClSUBFONT SIZE=-13/FONT/SUB, benzene, ether, and petroleum ether, and insoluble in acetone. At 20°C, synthetic PEs are insoluble (=1mg/100 ml of dry solvent) in acetone, ether, petroleum ether, and ethyl acetate; moderately soluble (20-100 mg/100 ml of dry solvent) in EtOH, pyridine, benzene, and CClSUBFONT SIZE=-14/FONT/SUB; readily soluble ( 1g/100 ml of solvent) in CHClSUBFONT SIZE=-13/FONT/SUB 2455/2456/2457.
	\|Mass Spectra=Negative ion mass spectrum during the elution of 1-stearol-2-arachidonyl-PE in a sample of rat liver PE. A Fisons VG Platform II single quadrupole mass spectrometer, fitted with an electrospray ion source operated at atmospheric pressure, was used for the identification of components eluting from the column. Nitrogen was used as nebulizer gas and as curtain gas. The capillary voltage was set to 3.0 kV and the cone voltage to 100 V {{Image200\|LBGPEccp:R:R:~~YS2ANe005:01SP0004~~.gif}} <<2462>>.,		\|Mass Spectra=Negative ion mass spectrum during the elution of 1-stearol-2-arachidonyl-PE in a sample of rat liver PE. A Fisons VG Platform II single quadrupole mass spectrometer, fitted with an electrospray ion source operated at atmospheric pressure, was used for the identification of components eluting from the column. Nitrogen was used as nebulizer gas and as curtain gas. The capillary voltage was set to 3.0 kV and the cone voltage to 100 V {{Image200\|LBGPEccp:R:R:YS2ANe005SP0004.gif}} 2462.,
	\|UV Spectra=Maximum absorbance at 205 nm <<2458>>.		\|UV Spectra=Maximum absorbance at 205 nm 2458.
	\|IR Spectra=Fourier transform IR spectra for dimyristoyl-PE in the dry state (anhydrous), either in the pure state (A, C and E) or in the presence of 20 mol% of <FONT FACE=~~"Symbol">a<~~/FONT>-tocopherol (B, D and F). Ester C=O stretching vibration mode region,1800-1700 ~~cm<SUP><FONT~~ SIZE=-1>-</FONT></~~SUP><SUP><FONT~~ SIZE=-~~1>1<~~/FONT></SUP>; ~~CH<SUB><FONT~~ SIZE=-~~1>2<~~/FONT></SUB> deformation, scissoring band region, 1500-1400 ~~cm<SUP><FONT~~ SIZE=-1>-</FONT></~~SUP><SUP><FONT~~ SIZE=-~~1>1<~~/FONT></SUP>; headgroup ~~PO<SUB><FONT~~ SIZE=-~~1>2<~~/FONT></~~SUB><SUP><FONT~~ SIZE=-1>-</FONT></SUP> phosphate band region, 1350-1150 ~~cm<SUP><FONT~~ SIZE=-1>-</FONT></~~SUP><SUP><FONT~~ SIZE=-~~1>1<~~/FONT></SUP> {{Image200\|LBGPEccp:R:R:~~YS2ANe005:01SP0001~~.gif}} <<2459>>.		\|IR Spectra=Fourier transform IR spectra for dimyristoyl-PE in the dry state (anhydrous), either in the pure state (A, C and E) or in the presence of 20 mol% of FONT FACE=Symbola/FONT-tocopherol (B, D and F). Ester C=O stretching vibration mode region,1800-1700 cmSUPFONT SIZE=-1-/FONT/SUPSUPFONT SIZE=-11/FONT/SUP; CHSUBFONT SIZE=-12/FONT/SUB deformation, scissoring band region, 1500-1400 cmSUPFONT SIZE=-1-/FONT/SUPSUPFONT SIZE=-11/FONT/SUP; headgroup POSUBFONT SIZE=-12/FONT/SUBSUPFONT SIZE=-1-/FONT/SUP phosphate band region, 1350-1150 cmSUPFONT SIZE=-1-/FONT/SUPSUPFONT SIZE=-11/FONT/SUP {{Image200\|LBGPEccp:R:R:YS2ANe005SP0001.gif}} 2459.
	\|NMR Spectra=~~<SUP><FONT~~ SIZE=-~~1>1<~~/FONT></~~SUP>H~~-NMR spectrum of PE. Varian VXR500 spectrometer operating at 500 MHz for protons, using ~~CHCl<SUB><FONT~~ SIZE=-~~1>3<~~/FONT></SUB> as the lock signal {{Image200\|LBGPEccp:R:R:~~YS2ANe005:01SP0002~~.gif}} <<2460>>./~~<BR><SUP><FONT~~ SIZE=-~~1>3<~~/FONT></~~SUP><SUP><FONT~~ SIZE=-~~1>1<~~/FONT></~~SUP>P~~ NMR spectrum of a PE (left) and a PC (right) fraction from bovine brain. Varian Unity 300 spectrometer operating at121.42 MHz using 1% ~~H<SUB><FONT~~ SIZE=-~~1>3<~~/FONT></~~SUB>PO<SUB><FONT~~ SIZE=-~~1>4<~~/FONT></SUB> as external standard {{Image200\|LBGPEccp:R:R:~~YS2ANe005:01SP0003~~.gif}} <<2461>>.,		\|NMR Spectra=SUPFONT SIZE=-11/FONT/SUPH-NMR spectrum of PE. Varian VXR500 spectrometer operating at 500 MHz for protons, using CHClSUBFONT SIZE=-13/FONT/SUB as the lock signal {{Image200\|LBGPEccp:R:R:YS2ANe005SP0002.gif}} 2460./BRSUPFONT SIZE=-13/FONT/SUPSUPFONT SIZE=-11/FONT/SUPP NMR spectrum of a PE (left) and a PC (right) fraction from bovine brain. Varian Unity 300 spectrometer operating at121.42 MHz using 1% HSUBFONT SIZE=-13/FONT/SUBPOSUBFONT SIZE=-14/FONT/SUB as external standard {{Image200\|LBGPEccp:R:R:YS2ANe005SP0003.gif}} 2461.,
	\|Chromatograms=2 dimensional TLC {{Image200\|LBGPEccp:R:R:~~YS2ANe005:01CH0001~~.gif}} <<2463>>/~~<BR>HPLC~~, Hewlett-Packard Model 1050 HPLC system, UV absorbance 205nm, Hewlett-Packard Model 3396 series II integrator, HP Hypersil C18 (200 x 2.1x 5 <FONT FACE=~~"Symbol">m<~~/~~FONT>m~~) column {{Image200\|LBGPEccp:R:R:~~YS2ANe005:01CH0002~~.gif}} <<2464>>/<BR> HPLC for intact PE molecular species. Molecular species of approxymately 50 nmol of PE were separated on two 250 x 4 mm Lichrospher RP-18 endcapped columns in series (Merck, Darmstadt, Germany). Isocratic elution was performed with a solvent consisting of acetonitrile-methanol 3:7 (v/v) containing 5 <FONT FACE=~~"Symbol">m<~~/~~FONT>M~~ ethanolamine, or a solvent consisiting of acetonitrile-methanol 2:3 (v/v) containing 2 <FONT FACE=~~"Symbol">m<~~/~~FONT>M~~ ethanolamine at a flow rate of 1.25 ml/min. The column effuluent was monitored at 206 nm using LKB 2251 Uvicord (Pharmacia, Upsala, Sweden) and subsequent ELSD was performed using a Varex MKIII obtained from Alltech, (Deerfield, IL) operating at a gas flow rate of 1.9 l/ml and a drift tube temperature of 100°C {{Image200\|LBGPEccp:R:R:~~YS2ANe005:01CH0003~~.gif}} {{Image200\|LBGPEccp:R:R:~~YS2ANe005:01FT0001~~.gif}} <<2462>>.,		\|Chromatograms=2 dimensional TLC {{Image200\|LBGPEccp:R:R:YS2ANe005CH0001.gif}} 2463/BRHPLC, Hewlett-Packard Model 1050 HPLC system, UV absorbance 205nm, Hewlett-Packard Model 3396 series II integrator, HP Hypersil C18 (200 x 2.1x 5 FONT FACE=Symbolm/FONTm) column {{Image200\|LBGPEccp:R:R:YS2ANe005CH0002.gif}} 2464/BR HPLC for intact PE molecular species. Molecular species of approxymately 50 nmol of PE were separated on two 250 x 4 mm Lichrospher RP-18 endcapped columns in series (Merck, Darmstadt, Germany). Isocratic elution was performed with a solvent consisting of acetonitrile-methanol 3:7 (v/v) containing 5 FONT FACE=Symbolm/FONTM ethanolamine, or a solvent consisiting of acetonitrile-methanol 2:3 (v/v) containing 2 FONT FACE=Symbolm/FONTM ethanolamine at a flow rate of 1.25 ml/min. The column effuluent was monitored at 206 nm using LKB 2251 Uvicord (Pharmacia, Upsala, Sweden) and subsequent ELSD was performed using a Varex MKIII obtained from Alltech, (Deerfield, IL) operating at a gas flow rate of 1.9 l/ml and a drift tube temperature of 100°C {{Image200\|LBGPEccp:R:R:YS2ANe005CH0003.gif}} {{Image200\|LBGPEccp:R:R:YS2ANe005FT0001.gif}} 2462.,
	}}		}}

	{{Lipid/Footer}}		{{Lipid/Footer}}

Editor: LBGPEccp:R:R:YS2ANe005:01 moved to LBGPEccp:R:R:YS2ANe005

2010-01-20T01:39:08Z

LBGPEccp:R:R:YS2ANe005:01 moved to LBGPEccp:R:R:YS2ANe005

← Older revision		Revision as of 10:39, 20 January 2010
(No difference)

Editor at 13:00, 26 July 2009

2009-07-26T13:00:00Z

← Older revision		Revision as of 22:00, 26 July 2009
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			{{Lipid/Header}}

	{{Metabolite		{{Metabolite
	\|LipidBank=PGP2410		\|LipidBank=PGP2410
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	\|Melting Point=196°C <<2455>>; dimyristoyl-PE, 175-177°C <<2456/2457>>; dipalmitoyl-PE, 172-175°C <<2456/2457>> 210-211°C <<2469>> dipalmitoyl-DL-, 211°C <<2469>>; distearoyl-PE, 172-173.5°C <<2456/2457>>; dioleoyl-PE, 195-200°C <<2469>>; dielaidoyl-PE, 193°C <<2469>>; 1-palmitoyl-2-oleoyl-PE, 194-196°C <<2471>>		\|Melting Point=196°C <<2455>>; dimyristoyl-PE, 175-177°C <<2456/2457>>; dipalmitoyl-PE, 172-175°C <<2456/2457>> 210-211°C <<2469>> dipalmitoyl-DL-, 211°C <<2469>>; distearoyl-PE, 172-173.5°C <<2456/2457>>; dioleoyl-PE, 195-200°C <<2469>>; dielaidoyl-PE, 193°C <<2469>>; 1-palmitoyl-2-oleoyl-PE, 194-196°C <<2471>>
	\|Reflactive=dimyristoyl-PE , [<FONT FACE="Symbol">a</FONT>]<sub>D</sub><sup>26</sup>+6.7° in CHCl<SUB><FONT SIZE=-1>3</FONT></SUB> <<2456/2457>>; dipalmitoyl-PE, [<FONT FACE="Symbol">a</FONT>]<sub>D</sub><sup>26</sup>+6.4° in CHCl<SUB><FONT SIZE=-1>3</FONT></SUB> <<2456/2457>>, [<FONT FACE="Symbol">a</FONT>]<sub>D</sub><sup>25</sup>+6.2° in CHCl<SUB><FONT SIZE=-1>3</FONT></SUB>-MeOH (2:1,v/v) <<2469>>; distearoyl-PE, [<FONT FACE="Symbol">a</FONT>]<sub>D</sub><sup>24</sup>+6.0° in CHCl<SUB><FONT SIZE=-1>3</FONT></SUB>-glacial acetic acid (9:1,v/v) <<2456/2457>>; dioleoyl-PE, [<FONT FACE="Symbol">a</FONT>]<sub>D</sub><sup>22</sup>+6.0° in CHCl<SUB><FONT SIZE=-1>3</FONT></SUB> <<2469>>; dielaidoyl-PE, [<FONT FACE="Symbol">a</FONT>]<sub>D</sub><sup>22</sup>+6.1° in CHCl<SUB><FONT SIZE=-1>3</FONT></SUB> <<2469>>; 1-palmitoyl-2-oleoyl-PE, [<FONT FACE="Symbol">a</FONT>]<sub>D</sub><sup>22</sup>+6.35° in CHCl<SUB><FONT SIZE=-1>3</FONT></SUB> <<2469>>		\|Reflactive=dimyristoyl-PE , [<FONT FACE="Symbol">a</FONT>]<sub>D</sub><sup>26</sup>+6.7° in CHCl<SUB><FONT SIZE=-1>3</FONT></SUB> <<2456/2457>>; dipalmitoyl-PE, [<FONT FACE="Symbol">a</FONT>]<sub>D</sub><sup>26</sup>+6.4° in CHCl<SUB><FONT SIZE=-1>3</FONT></SUB> <<2456/2457>>, [<FONT FACE="Symbol">a</FONT>]<sub>D</sub><sup>25</sup>+6.2° in CHCl<SUB><FONT SIZE=-1>3</FONT></SUB>-MeOH (2:1,v/v) <<2469>>; distearoyl-PE, [<FONT FACE="Symbol">a</FONT>]<sub>D</sub><sup>24</sup>+6.0° in CHCl<SUB><FONT SIZE=-1>3</FONT></SUB>-glacial acetic acid (9:1,v/v) <<2456/2457>>; dioleoyl-PE, [<FONT FACE="Symbol">a</FONT>]<sub>D</sub><sup>22</sup>+6.0° in CHCl<SUB><FONT SIZE=-1>3</FONT></SUB> <<2469>>; dielaidoyl-PE, [<FONT FACE="Symbol">a</FONT>]<sub>D</sub><sup>22</sup>+6.1° in CHCl<SUB><FONT SIZE=-1>3</FONT></SUB> <<2469>>; 1-palmitoyl-2-oleoyl-PE, [<FONT FACE="Symbol">a</FONT>]<sub>D</sub><sup>22</sup>+6.35° in CHCl<SUB><FONT SIZE=-1>3</FONT></SUB> <<2469>>
	\|Solubility=Insoluble in water. Natural PE is soluble in MeOH, EtOH, CHCl<SUB><FONT SIZE=-1>3</FONT></SUB>, benzene, ether, and petroleum ether, and insoluble in acetone. At 20°C, synthetic PEs are insoluble (=1mg/100 ml of dry solvent) in acetone, ether, petroleum ether, and ethyl acetate; moderately soluble (20-100 mg/100 ml of dry solvent) in EtOH, pyridine, benzene, and CCl<SUB><FONT SIZE=-1>4</FONT></SUB>; readily soluble (> 1g/100 ml of solvent) in CHCl<SUB><FONT SIZE=-1>3</FONT></SUB> <<2455/2456/2457>>.		\|Solubility=Insoluble in water. Natural PE is soluble in MeOH, EtOH, CHCl<SUB><FONT SIZE=-1>3</FONT></SUB>, benzene, ether, and petroleum ether, and insoluble in acetone. At 20°C, synthetic PEs are insoluble (<=1mg/100 ml of dry solvent) in acetone, ether, petroleum ether, and ethyl acetate; moderately soluble (20-100 mg/100 ml of dry solvent) in EtOH, pyridine, benzene, and CCl<SUB><FONT SIZE=-1>4</FONT></SUB>; readily soluble (> 1g/100 ml of solvent) in CHCl<SUB><FONT SIZE=-1>3</FONT></SUB> <<2455/2456/2457>>.
	\|Mass Spectra=Negative ion mass spectrum during the elution of 1-stearol-2-arachidonyl-PE in a sample of rat liver PE. A Fisons VG Platform II single quadrupole mass spectrometer, fitted with an electrospray ion source operated at atmospheric pressure, was used for the identification of components eluting from the column. Nitrogen was used as nebulizer gas and as curtain gas. The capillary voltage was set to 3.0 kV and the cone voltage to 100 V {{Image200\|LBGPEccp:R:R:YS2ANe005:01SP0004.gif}} <<2462>>.,		\|Mass Spectra=Negative ion mass spectrum during the elution of 1-stearol-2-arachidonyl-PE in a sample of rat liver PE. A Fisons VG Platform II single quadrupole mass spectrometer, fitted with an electrospray ion source operated at atmospheric pressure, was used for the identification of components eluting from the column. Nitrogen was used as nebulizer gas and as curtain gas. The capillary voltage was set to 3.0 kV and the cone voltage to 100 V {{Image200\|LBGPEccp:R:R:YS2ANe005:01SP0004.gif}} <<2462>>.,
	\|UV Spectra=Maximum absorbance at 205 nm <<2458>>.		\|UV Spectra=Maximum absorbance at 205 nm <<2458>>.
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	\|Chromatograms=2 dimensional TLC {{Image200\|LBGPEccp:R:R:YS2ANe005:01CH0001.gif}} <<2463>>/<BR>HPLC, Hewlett-Packard Model 1050 HPLC system, UV absorbance 205nm, Hewlett-Packard Model 3396 series II integrator, HP Hypersil C18 (200 x 2.1x 5 <FONT FACE="Symbol">m</FONT>m) column {{Image200\|LBGPEccp:R:R:YS2ANe005:01CH0002.gif}} <<2464>>/<BR> HPLC for intact PE molecular species. Molecular species of approxymately 50 nmol of PE were separated on two 250 x 4 mm Lichrospher RP-18 endcapped columns in series (Merck, Darmstadt, Germany). Isocratic elution was performed with a solvent consisting of acetonitrile-methanol 3:7 (v/v) containing 5 <FONT FACE="Symbol">m</FONT>M ethanolamine, or a solvent consisiting of acetonitrile-methanol 2:3 (v/v) containing 2 <FONT FACE="Symbol">m</FONT>M ethanolamine at a flow rate of 1.25 ml/min. The column effuluent was monitored at 206 nm using LKB 2251 Uvicord (Pharmacia, Upsala, Sweden) and subsequent ELSD was performed using a Varex MKIII obtained from Alltech, (Deerfield, IL) operating at a gas flow rate of 1.9 l/ml and a drift tube temperature of 100°C {{Image200\|LBGPEccp:R:R:YS2ANe005:01CH0003.gif}} {{Image200\|LBGPEccp:R:R:YS2ANe005:01FT0001.gif}} <<2462>>.,		\|Chromatograms=2 dimensional TLC {{Image200\|LBGPEccp:R:R:YS2ANe005:01CH0001.gif}} <<2463>>/<BR>HPLC, Hewlett-Packard Model 1050 HPLC system, UV absorbance 205nm, Hewlett-Packard Model 3396 series II integrator, HP Hypersil C18 (200 x 2.1x 5 <FONT FACE="Symbol">m</FONT>m) column {{Image200\|LBGPEccp:R:R:YS2ANe005:01CH0002.gif}} <<2464>>/<BR> HPLC for intact PE molecular species. Molecular species of approxymately 50 nmol of PE were separated on two 250 x 4 mm Lichrospher RP-18 endcapped columns in series (Merck, Darmstadt, Germany). Isocratic elution was performed with a solvent consisting of acetonitrile-methanol 3:7 (v/v) containing 5 <FONT FACE="Symbol">m</FONT>M ethanolamine, or a solvent consisiting of acetonitrile-methanol 2:3 (v/v) containing 2 <FONT FACE="Symbol">m</FONT>M ethanolamine at a flow rate of 1.25 ml/min. The column effuluent was monitored at 206 nm using LKB 2251 Uvicord (Pharmacia, Upsala, Sweden) and subsequent ELSD was performed using a Varex MKIII obtained from Alltech, (Deerfield, IL) operating at a gas flow rate of 1.9 l/ml and a drift tube temperature of 100°C {{Image200\|LBGPEccp:R:R:YS2ANe005:01CH0003.gif}} {{Image200\|LBGPEccp:R:R:YS2ANe005:01FT0001.gif}} <<2462>>.,
	}}		}}

			{{Lipid/Footer}}

Editor at 12:00, 8 July 2009

2009-07-08T12:00:00Z

New page

{{Metabolite
|LipidBank=PGP2410
|SysName=L-a-phosphatidylethanolamine
|Common Name=&&phosphatidylethanolamine&& cephalin&& ethanolaminephosphoglyceride&& (3-sn-phosphatidyl) ethanolamine &&L-a-phosphatidylethanolamine&&
|Melting Point=196°C <<2455>>; dimyristoyl-PE, 175-177°C <<2456/2457>>; dipalmitoyl-PE, 172-175°C <<2456/2457>> 210-211°C <<2469>> dipalmitoyl-DL-, 211°C <<2469>>; distearoyl-PE, 172-173.5°C <<2456/2457>>; dioleoyl-PE, 195-200°C <<2469>>; dielaidoyl-PE, 193°C <<2469>>; 1-palmitoyl-2-oleoyl-PE, 194-196°C <<2471>>
|Reflactive=dimyristoyl-PE , [a]D26+6.7° in CHCl3 <<2456/2457>>; dipalmitoyl-PE, [a]D26+6.4° in CHCl3 <<2456/2457>>, [a]D25+6.2° in CHCl3-MeOH (2:1,v/v) <<2469>>; distearoyl-PE, [a]D24+6.0° in CHCl3-glacial acetic acid (9:1,v/v) <<2456/2457>>; dioleoyl-PE, [a]D22+6.0° in CHCl3 <<2469>>; dielaidoyl-PE, [a]D22+6.1° in CHCl3 <<2469>>; 1-palmitoyl-2-oleoyl-PE, [a]D22+6.35° in CHCl3 <<2469>>
|Solubility=Insoluble in water. Natural PE is soluble in MeOH, EtOH, CHCl3, benzene, ether, and petroleum ether, and insoluble in acetone. At 20°C, synthetic PEs are insoluble (=1mg/100 ml of dry solvent) in acetone, ether, petroleum ether, and ethyl acetate; moderately soluble (20-100 mg/100 ml of dry solvent) in EtOH, pyridine, benzene, and CCl4; readily soluble (> 1g/100 ml of solvent) in CHCl3 <<2455/2456/2457>>.
|Mass Spectra=Negative ion mass spectrum during the elution of 1-stearol-2-arachidonyl-PE in a sample of rat liver PE. A Fisons VG Platform II single quadrupole mass spectrometer, fitted with an electrospray ion source operated at atmospheric pressure, was used for the identification of components eluting from the column. Nitrogen was used as nebulizer gas and as curtain gas. The capillary voltage was set to 3.0 kV and the cone voltage to 100 V {{Image200|LBGPEccp:R:R:YS2ANe005:01SP0004.gif}} <<2462>>.,
|UV Spectra=Maximum absorbance at 205 nm <<2458>>.
|IR Spectra=Fourier transform IR spectra for dimyristoyl-PE in the dry state (anhydrous), either in the pure state (A, C and E) or in the presence of 20 mol% of a-tocopherol (B, D and F). Ester C=O stretching vibration mode region,1800-1700 cm-1; CH2 deformation, scissoring band region, 1500-1400 cm-1; headgroup PO2- phosphate band region, 1350-1150 cm-1 {{Image200|LBGPEccp:R:R:YS2ANe005:01SP0001.gif}} <<2459>>.
|NMR Spectra=1H-NMR spectrum of PE. Varian VXR500 spectrometer operating at 500 MHz for protons, using CHCl3 as the lock signal {{Image200|LBGPEccp:R:R:YS2ANe005:01SP0002.gif}} <<2460>>./ 31P NMR spectrum of a PE (left) and a PC (right) fraction from bovine brain. Varian Unity 300 spectrometer operating at121.42 MHz using 1% H3PO4 as external standard {{Image200|LBGPEccp:R:R:YS2ANe005:01SP0003.gif}} <<2461>>.,
|Chromatograms=2 dimensional TLC {{Image200|LBGPEccp:R:R:YS2ANe005:01CH0001.gif}} <<2463>>/ HPLC, Hewlett-Packard Model 1050 HPLC system, UV absorbance 205nm, Hewlett-Packard Model 3396 series II integrator, HP Hypersil C18 (200 x 2.1x 5 mm) column {{Image200|LBGPEccp:R:R:YS2ANe005:01CH0002.gif}} <<2464>>/ HPLC for intact PE molecular species. Molecular species of approxymately 50 nmol of PE were separated on two 250 x 4 mm Lichrospher RP-18 endcapped columns in series (Merck, Darmstadt, Germany). Isocratic elution was performed with a solvent consisting of acetonitrile-methanol 3:7 (v/v) containing 5 mM ethanolamine, or a solvent consisiting of acetonitrile-methanol 2:3 (v/v) containing 2 mM ethanolamine at a flow rate of 1.25 ml/min. The column effuluent was monitored at 206 nm using LKB 2251 Uvicord (Pharmacia, Upsala, Sweden) and subsequent ELSD was performed using a Varex MKIII obtained from Alltech, (Deerfield, IL) operating at a gas flow rate of 1.9 l/ml and a drift tube temperature of 100°C {{Image200|LBGPEccp:R:R:YS2ANe005:01CH0003.gif}} {{Image200|LBGPEccp:R:R:YS2ANe005:01FT0001.gif}} <<2462>>.,
}}