Category:LBS/Protocol/Separation: Difference between revisions
(Created page with "{| | {| class="wikitable" style="text-align:center" ! colspan="3"| '''DEAE-Sephadex''' (weak anion exchanger) |- |rowspan="3" valign="top"|adsorb <br/>↓<br/>'''acidic''' fra...") |
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{| class="wikitable" style="text-align:center" | {| class="wikitable" style="text-align:center" | ||
! colspan=" | ! colspan="4"|'''QAE-Sephadex''' (strong anion exchanger) | ||
|- style="vertical-align:top" | |||
|colspan="2"|not adsorb (C/M/W fraction)<br/>↓<br/>acetylation | |||
|colspan="2"|adsorb (ammonium acetate fraction)<br/>↓<br/> | |||
|- | |- | ||
| | !colspan="2"| '''Florisil''' (Mg silicate) | ||
!colspan="2"| '''DEAE-Sephadex''' (weak anion exchanger) | |||
|- style="vertical-align:top" | |||
| not adsorb<br/>↓<br/>'''neutral''' fraction<br/>others | |||
| adsorb<br/>↓<br/>'''twitterionic''' fraction<br/>sphingomyelins | |||
| not adsorb<br/>↓<br/> '''polar''' fraction<br/>ceramide aminoetylphosphonate (CAEPn) | |||
| adsorb<br/>↓<br/> '''acidic''' fraction<br/>[[File:LBS-Monomer-NeuAc.png]][[File:LBS-Monomer-NeuGc.png]][[File:LBS-Monomer-KDN.png]][[File:LBS-Monomer-GlcA.png]][[File:LBS-Monomer-GalA.png]]<small>(sialic acids and uronate)</small><br/>[[File:LBS-Monomer-P.png]][[File:LBS-Monomer-S.png]]<small>(sulfate and phosphate)</small> | |||
|} | |} | ||
Latest revision as of 12:18, 6 December 2015
| QAE-Sephadex (strong anion exchanger) | |||
|---|---|---|---|
| not adsorb (C/M/W fraction) ↓ acetylation |
adsorb (ammonium acetate fraction) ↓ | ||
| Florisil (Mg silicate) | DEAE-Sephadex (weak anion exchanger) | ||
| not adsorb ↓ neutral fraction others |
adsorb ↓ twitterionic fraction sphingomyelins |
not adsorb ↓ polar fraction ceramide aminoetylphosphonate (CAEPn) |
adsorb ↓ acidic fraction      (sialic acids and uronate)  (sulfate and phosphate)
|
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