View source for Taguchi:Abbreviations

==Materials==
{| class="wikitable sortable"
|-
! Abbreviation || Description
{{#repeat:Taguchi/Table/Row2|1|
&&Ace && acetone
&&AceA && acetic acid
&&ACN && acetonitrile
&&Amm && ammonia
&&AmFo && ammonium formate
&&But && butanol
&&Chl && chloroform
&&ForA && formic acid
&&Hex && hexane
&&IsoP && isopropanol
&&Met && methanol
&&MFo && methyl formate
&&NaAc && sodium acetate
&&PhoA && phosphoric acid
&&TriE && triethylamine
&&W && water
}}
|}

==Extraction Methods==
{| class="wikitable sortable"
|-
! Abbreviation || Description
{{#repeat:Taguchi/Table/Row2|1|
&&B&D && Bligh and Dyer method
&&SPE && Solid-phase extraction method
}}
|}

==Purification==
{| class="wikitable sortable"
|-
! Abbreviation || Description || Vendor
{{#repeat:Taguchi/Table/Row3|1|
&& C18 SP && C18 Sep-Pak && Waters
&& DEAEc && DEAE cellulose && Wako Pure Chemicals
&& DEAEsA25 && EAE sephadex A-25 && Amersham Biosciences
&& GS4B && glutathione Sepharose 4B && Amersham Biosciences
&& SiG && Silica gel &&
&& OHLB && Oasis HLB && Waters
}}
|}

==Parameters==
{| class="wikitable sortable"
|-
! Abbreviation || Description
{{#repeat:Taguchi/Table/Row2|1|
&&Me  && Methanol
&&CM1:1 && methanol(2ml), chloroform(2ml), 1 N HCl(2ml), 2 M NaCl(0.15ml)
&&CM2:1 && chloroform-methanol (2:1; v/v)
&&CMW1:2:0.8 && chloroform-methanol-water (1:2:0.8; v/v/v)
&&CuSO4 && ???
&&TX-114 && ???
&&SiGel&& chloroform, acetone, chloroform/methanol (7:3)
&&SiTLC && chloroform/methanol/water  (65:25:4)
&&WCM && water chloroform methanol
&&WHM && water hexane methanol
&&AMW18:11:1&& acetonitrile/methanol/water 18:11:1 (0.1% ammonium formate, pH 7.8)
&&CM200:100:3&& chloroform chloroform/methanol(1:1) chloroform/methanol/28% aqueous ammonia/acetic acid (200:100:3:0.9) chloroform/methanol/HCl (6:6:1)
&&CMW30:60:8&& chloroform-methanol-water (30:60:8; v/v/v) chloroform-methanol-0.8 M sodium acetate (30:60:8; v/v/v)
&&GSH&& 20 mM reduced glutathiglutathione, 30 mM Tris, and 150 mM NaCl
}}
|}

==Gradient Programs==
===Mobile phase A===
{| class="wikitable sortable"
|-
! Abbreviation || Description
{{#repeat:Taguchi/Table/Row2|1|
&&AMW19:19:2 && acetonitrile/methanol/water:19/19/2 (0.1% acetic acid + 0.028% ammonia)
&&AMW6:7:2 &&   acetonitrile/methanol/water:6/7/2 (0.1% ammonium formate pH 7.0)
&&AMW18:11:1 && acetonitrile/methanol/water 18:11:1 (0.1% ammonium formate, pH 7.8)
&&AMW2:2:1 && acetoniacetonitrile:methanol:water 2:2:1 v/v/v containing 0.1% formic acid and 0.028% ammonia
&&MW1000:1 &&   methanol-28% aqueous ammonium-water (1000 : 1 : 1, pH 11)
&&MW50:50 &&  methanol/water/70% ethylamine (50:50:0.13)
&&AM2:1 &&      acetonitrile–methanol (2 : 1) containing 0.1% ammonium formate (pH 6.4)
&&AMEa25:75:1 && acetonitrile/methanol/triethylamine 25:75:1 (0.3% formic acid, pH 6.8)
&&AW5:95 &&     acetonitrile/water (5:95) containing 0.05% formic acid
&&PMW5:1:4 &&   isopropanol/methanol/water (5:1:4) supplemented with 0.2% formic acid, 0.028% ammonia, and 5 micro M phosphoric acid
&&PMW25:55:20 && methanol (A), isopropanol (B), and water (containing 200 mM ammonium formate) (C), (A/B/C 55:25:20)
}}
|}

===Mobile phase B===
{| class="wikitable sortable"
|-
! Abbreviation || Description
{{#repeat:Taguchi/Table/Row2|1|
&&PAc0.1 && isopropanol (0.1% acetic acid + 0.028% ammonia)
&&PFa0.2 && isopropanol supplemented with 0.2% formic acid, 0.028% ammonia
&&PFa0.1 && isopropanol containing 0.1% formic acid and 0.028% ammonia
&&PEa0.13 && 2-propanol/70% ethylamine (100:0.13)
&&MW2:1 && methanol–H2O (2 : 1) containing 0.1% ammonium formate (pH 6.4)
&&PMW40:60:? && methanol (A), isopropanol (B), and water (containing 200 mM ammonium formate) (C), (A/B 60:40)
&&AW90:10 && acetonitrile/H2O (90:10) containing 0.05% formic acid
}}
|}

===Gradient program===
{| class="wikitable sortable"
|-
! Abbreviation || Description
{{#repeat:Taguchi/Table/Row2|1|
&&IE && isocratic elution A:B 1:1 v/v
&&90/10:60/40:45/55 && holding solvent (A/B:90/10) for 5 min linearly converting to solvent (A/B:60/40) for 35min linearly converting solvent (A/B:45/55) for 50 min
&&100/0:70/30:0/100 && 100% A for 10 min, increased 30%B at 60 min, 100%B for 15 min
&&95/5:10/90 && 95%/5% (A/B) to 10%/90% (A/B)
&&100/0:50/50:100/0 && 100:0 (0–5 min), 50:50(5–25 min), 50:50 (25–59 min), 100:0 (59–60 min), and 100:0(60–75 min)
&&100/0:95/5:40/60 && 100% A for 0–10 min linear gradient from 95%A/5% B to 40% A/60% B for 10–110 min
}}
|}