Category:LBS/Protocol/Separation: Difference between revisions

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{| class="wikitable" style="text-align:center"
{| class="wikitable" style="text-align:center"
! colspan="3"| '''DEAE-Sephadex''' (weak anion exchanger)
! colspan="4"|'''QAE-Sephadex''' (strong anion exchanger)  
|- style="vertical-align:top"
|colspan="2"|not adsorb (C/M/W fraction)<br/>↓<br/>acetylation
|colspan="2"|adsorb (ammonium acetate fraction)<br/>↓<br/>
|-
|-
|rowspan="3" valign="top"|adsorb <br/>↓<br/>'''acidic''' fraction<br/>[[File:LBS-Monomer-NeuAc.png]][[File:LBS-Monomer-NeuGc.png]][[File:LBS-Monomer-KDN.png]][[File:LBS-Monomer-GlcA.png]][[File:LBS-Monomer-GalA.png]][[File:LBS-Monomer-P.png]][[File:LBS-Monomer-S.png]]
!colspan="2"| '''Florisil''' (Mg silicate)
|colspan="2"| not adsorb<br/>↓
!colspan="2"| '''DEAE-Sephadex''' (weak anion exchanger)
|-
|- style="vertical-align:top"
!colspan="2"|'''QAE-Sephadex''' (strong anion exchanger)
| not adsorb<br/>↓<br/>'''neutral''' fraction<br/>others
|-
| adsorb<br/>↓<br/>'''twitterionic''' fraction<br/>sphingomyelins
| adsorb <br/>↓<br/>'''polar''' fraction<br/>phosphoethanolamine
| not adsorb<br/>↓<br/> '''polar''' fraction<br/>ceramide aminoetylphosphonate (CAEPn)
|not adsorb<br/>↓<br/>'''neutral''' fraction<br/>
| adsorb<br/>↓<br/> '''acidic''' fraction<br/>[[File:LBS-Monomer-NeuAc.png]][[File:LBS-Monomer-NeuGc.png]][[File:LBS-Monomer-KDN.png]][[File:LBS-Monomer-GlcA.png]][[File:LBS-Monomer-GalA.png]]<small>(sialic acids and uronate)</small><br/>[[File:LBS-Monomer-P.png]][[File:LBS-Monomer-S.png]]<small>(sulfate and phosphate)</small>
|}
|}

Latest revision as of 21:18, 6 December 2015

QAE-Sephadex (strong anion exchanger)
not adsorb (C/M/W fraction)
↓
acetylation
adsorb (ammonium acetate fraction)
↓
Florisil (Mg silicate) DEAE-Sephadex (weak anion exchanger)
not adsorb
↓
neutral fraction
others
adsorb
↓
twitterionic fraction
sphingomyelins
not adsorb
↓
polar fraction
ceramide aminoetylphosphonate (CAEPn)
adsorb
↓
acidic fraction
(sialic acids and uronate)
(sulfate and phosphate)

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