Taguchi:Abbreviations

Extraction Methods

Abbreviation	Description
B&D	Bligh and Dyer method
Me	Methanol
CM1:1	methanol(2ml), chloroform(2ml), 1 N HCl(2ml), 2 M NaCl(0.15ml)
CM2:1	chloroform-methanol (2:1; v/v)
CMW1:2:0.8	chloroform-methanol-water (1:2:0.8; v/v/v)
CuSO4	???
TX-114	???
SPE	Solid Phase extraction
SiGel	chloroform, acetone, chloroform/methanol (7:3)
SiTLC	chloroform/methanol/water (65:25:4)
WCM	water chloroform methanol
WHM	water hexane methanol
AMW18:11:1	acetonitrile/methanol/water 18:11:1 (0.1% ammonium formate, pH 7.8)
CM200:100:3	chloroform chloroform/methanol(1:1) chloroform/methanol/28% aqueous ammonia/acetic acid (200:100:3:0.9) chloroform/methanol/HCl (6:6:1)
CMW30:60:8	chloroform-methanol-water (30:60:8; v/v/v) chloroform-methanol-0.8 M sodium acetate (30:60:8; v/v/v)
GSH	20 mM reduced glutathiglutathione, 30 mM Tris, and 150 mM NaCl

Gradient Programs

Mobile phase A

Abbreviation	Description
AMW19:19:2	acetonitrile/methanol/water:19/19/2 (0.1% acetic acid + 0.028% ammonia)
AMW6:7:2	acetonitrile/methanol/water:6/7/2 (0.1% ammonium formate pH 7.0)
AMW18:11:1	acetonitrile/methanol/water 18:11:1 (0.1% ammonium formate, pH 7.8)
AMW2:2:1	acetoniacetonitrile:methanol:water 2:2:1 v/v/v containing 0.1% formic acid and 0.028% ammonia
MW1000:1	methanol-28% aqueous ammonium-water (1000 : 1 : 1, pH 11)
MW50:50	methanol/water/70% ethylamine (50:50:0.13)
AM2:1	acetonitrile–methanol (2 : 1) containing 0.1% ammonium formate (pH 6.4)
AMEa25:75:1	acetonitrile/methanol/triethylamine 25:75:1 (0.3% formic acid, pH 6.8)
AW5:95	acetonitrile/water (5:95) containing 0.05% formic acid
PMW5:1:4	isopropanol/methanol/water (5:1:4) supplemented with 0.2% formic acid, 0.028% ammonia, and 5 micro M phosphoric acid
PMW25:55:20	methanol (A), isopropanol (B), and water (containing 200 mM ammonium formate) (C), (A/B/C 55:25:20)

Mobile phase B

Abbreviation	Description
PAc0.1	isopropanol (0.1% acetic acid + 0.028% ammonia)
PFa0.2	isopropanol supplemented with 0.2% formic acid, 0.028% ammonia
PFa0.1	isopropanol containing 0.1% formic acid and 0.028% ammonia
PEa0.13	2-propanol/70% ethylamine (100:0.13)
MW2:1	methanol–H2O (2 : 1) containing 0.1% ammonium formate (pH 6.4)
PMW40:60:?	methanol (A), isopropanol (B), and water (containing 200 mM ammonium formate) (C), (A/B 60:40)
AW90:10	acetonitrile/H2O (90:10) containing 0.05% formic acid

Gradient program

Abbreviation	Description
IE	isocratic elution A:B 1:1 v/v
90/10:60/40:45/55	holding solvent (A/B:90/10) for 5 min linearly converting to solvent (A/B:60/40) for 35min linearly converting solvent (A/B:45/55) for 50 min
100/0:70/30:0/100	100% A for 10 min, increased 30%B at 60 min, 100%B for 15 min
95/5:10/90	95%/5% (A/B) to 10%/90% (A/B)
100/0:50/50:100/0	100:0 (0–5 min), 50:50(5–25 min), 50:50 (25–59 min), 100:0 (59–60 min), and 100:0(60–75 min)
100/0:95/5:40/60	100% A for 0–10 min linear gradient from 95%A/5% B to 40% A/60% B for 10–110 min