Taguchi:Abbreviations

Materials

Abbreviation	Description
Ace	acetone
AceA	acetic acid
ACN	acetonitrile
Amm	ammonia
AmFo	ammonium formate
But	butanol
Chl	chloroform
ForA	formic acid
Hex	hexane
IsoP	isopropanol
Met	methanol
MFo	methyl formate
NaAc	sodium acetate
PhoA	phosphoric acid
TriE	triethylamine
W	water

Extraction Methods

Abbreviation	Description
B&D	Bligh and Dyer method
SPE	Solid-phase extraction method

Purification

Abbreviation	Description	Vendor
C18 SP	C18 Sep-Pak	Waters
DEAEc	DEAE cellulose	Wako Pure Chemicals
DEAEsA25	EAE sephadex A-25	Amersham Biosciences
GS4B	glutathione Sepharose 4B	Amersham Biosciences
SiG	Silica gel
OHLB	Oasis HLB	Waters

Parameters

Abbreviation	Description
Me	Methanol
CM1:1	methanol(2ml), chloroform(2ml), 1 N HCl(2ml), 2 M NaCl(0.15ml)
CM2:1	chloroform-methanol (2:1; v/v)
CMW1:2:0.8	chloroform-methanol-water (1:2:0.8; v/v/v)
CuSO4	???
TX-114	???
SiGel	chloroform, acetone, chloroform/methanol (7:3)
SiTLC	chloroform/methanol/water (65:25:4)
WCM	water chloroform methanol
WHM	water hexane methanol
AMW18:11:1	acetonitrile/methanol/water 18:11:1 (0.1% ammonium formate, pH 7.8)
CM200:100:3	chloroform chloroform/methanol(1:1) chloroform/methanol/28% aqueous ammonia/acetic acid (200:100:3:0.9) chloroform/methanol/HCl (6:6:1)
CMW30:60:8	chloroform-methanol-water (30:60:8; v/v/v) chloroform-methanol-0.8 M sodium acetate (30:60:8; v/v/v)
GSH	20 mM reduced glutathiglutathione, 30 mM Tris, and 150 mM NaCl

Gradient Programs

Mobile phase A

Abbreviation	Description
AMW19:19:2	acetonitrile/methanol/water:19/19/2 (0.1% acetic acid + 0.028% ammonia)
AMW6:7:2	acetonitrile/methanol/water:6/7/2 (0.1% ammonium formate pH 7.0)
AMW18:11:1	acetonitrile/methanol/water 18:11:1 (0.1% ammonium formate, pH 7.8)
AMW2:2:1	acetoniacetonitrile:methanol:water 2:2:1 v/v/v containing 0.1% formic acid and 0.028% ammonia
MW1000:1	methanol-28% aqueous ammonium-water (1000 : 1 : 1, pH 11)
MW50:50	methanol/water/70% ethylamine (50:50:0.13)
AM2:1	acetonitrile–methanol (2 : 1) containing 0.1% ammonium formate (pH 6.4)
AMEa25:75:1	acetonitrile/methanol/triethylamine 25:75:1 (0.3% formic acid, pH 6.8)
AW5:95	acetonitrile/water (5:95) containing 0.05% formic acid
PMW5:1:4	isopropanol/methanol/water (5:1:4) supplemented with 0.2% formic acid, 0.028% ammonia, and 5 micro M phosphoric acid
PMW25:55:20	methanol (A), isopropanol (B), and water (containing 200 mM ammonium formate) (C), (A/B/C 55:25:20)

Mobile phase B

Abbreviation	Description
PAc0.1	isopropanol (0.1% acetic acid + 0.028% ammonia)
PFa0.2	isopropanol supplemented with 0.2% formic acid, 0.028% ammonia
PFa0.1	isopropanol containing 0.1% formic acid and 0.028% ammonia
PEa0.13	2-propanol/70% ethylamine (100:0.13)
MW2:1	methanol–H2O (2 : 1) containing 0.1% ammonium formate (pH 6.4)
PMW40:60:?	methanol (A), isopropanol (B), and water (containing 200 mM ammonium formate) (C), (A/B 60:40)
AW90:10	acetonitrile/H2O (90:10) containing 0.05% formic acid

Gradient program

Abbreviation	Description
IE	isocratic elution A:B 1:1 v/v
90/10:60/40:45/55	holding solvent (A/B:90/10) for 5 min linearly converting to solvent (A/B:60/40) for 35min linearly converting solvent (A/B:45/55) for 50 min
100/0:70/30:0/100	100% A for 10 min, increased 30%B at 60 min, 100%B for 15 min
95/5:10/90	95%/5% (A/B) to 10%/90% (A/B)
100/0:50/50:100/0	100:0 (0–5 min), 50:50(5–25 min), 50:50 (25–59 min), 100:0 (59–60 min), and 100:0(60–75 min)
100/0:95/5:40/60	100% A for 0–10 min linear gradient from 95%A/5% B to 40% A/60% B for 10–110 min