Taguchi:Abbreviations

Materials

Abbreviation	Description
Ace	acetone
AceA	acetic acid
ACN	acetonitrile
Amm	ammonia
AmFo	ammonium formate
But	butanol
Chl	chloroform
ForA	formic acid
Hex	hexane
IsoP	isopropanol
Met	methanol
MFo	methyl formate
NaAc	sodium acetate
PhoA	phosphoric acid
TriE	triethylamine
W	water

Extraction Methods

Abbreviation	Description
B&D	Bligh and Dyer method
SPE	Solid-phase extraction method
TLC	Thin layer chromatography

Purification

Abbreviation	Description	Vendor
C18 SP	C18 Sep-Pak	Waters
DEAEc	DEAE cellulose	Wako Pure Chemicals
DEAEsA25	EAE sephadex A-25	Amersham Biosciences
GS4B	glutathione Sepharose 4B	Amersham Biosciences
SiG	Silica gel
OHLB	Oasis HLB	Waters

Liquid Chromatography

Phase

Abbreviation	Description
Nor	normal
Rev	reverse

Program

Abbreviation	Description
Gra	gradient
Iso	isocratic
Lin	linear
Ste	stepwise

Column

Abbreviation	Description	Vendor
ACU18	ACQUITY UPLC BEH C18	Waters
APC18	Acclaim PepMap300 C18	LC PACKINGS
DevC30	Deverosil C30	Nomura Chemicals
DevSi60	Deverosil Si60	Nomura Chemicals
HiQ sil	HiQ sil	KYA TECH
Si60	Si60	Nomura Chemicals
UC18	Unison C18 nano LC column	Imtakt
WXBC18	Waters X-Bridge C18	Waters

System

Abbreviation	Description	Vendor
AU	ACQUITY Ultra Performance LC system	Waters
HP1100	HP 1100 HPLC system	Hewlett-Packard
LC10AD	LC-10AD vp micro HPLC system	Shimadzu
P305	pump model 305	Gilson
UM	Ultimate HPLC system	LC Packings
UM3000	UltiMate 3000 nano/cap/micro LC system	Dionex

Program

Abbreviation	Description
Gra	gradient
Iso	isocratic
Lin	linear
Ste	stepwise

Parameters

Abbreviation	Description
Me	Methanol
CM1:1	methanol(2ml), chloroform(2ml), 1 N HCl(2ml), 2 M NaCl(0.15ml)
CM2:1	chloroform-methanol (2:1; v/v)
CMW1:2:0.8	chloroform-methanol-water (1:2:0.8; v/v/v)
CuSO4	???
TX-114	???
SiGel	chloroform, acetone, chloroform/methanol (7:3)
SiTLC	chloroform/methanol/water (65:25:4)
WCM	water chloroform methanol
WHM	water hexane methanol
AMW18:11:1	acetonitrile/methanol/water 18:11:1 (0.1% ammonium formate, pH 7.8)
CM200:100:3	chloroform chloroform/methanol(1:1) chloroform/methanol/28% aqueous ammonia/acetic acid (200:100:3:0.9) chloroform/methanol/HCl (6:6:1)
CMW30:60:8	chloroform-methanol-water (30:60:8; v/v/v) chloroform-methanol-0.8 M sodium acetate (30:60:8; v/v/v)
GSH	20 mM reduced glutathiglutathione, 30 mM Tris, and 150 mM NaCl

Gradient Programs

Mobile phase A

Abbreviation	Description
AMW19:19:2	acetonitrile/methanol/water:19/19/2 (0.1% acetic acid + 0.028% ammonia)
AMW6:7:2	acetonitrile/methanol/water:6/7/2 (0.1% ammonium formate pH 7.0)
AMW18:11:1	acetonitrile/methanol/water 18:11:1 (0.1% ammonium formate, pH 7.8)
AMW2:2:1	acetoniacetonitrile:methanol:water 2:2:1 v/v/v containing 0.1% formic acid and 0.028% ammonia
MW1000:1	methanol-28% aqueous ammonium-water (1000 : 1 : 1, pH 11)
MW50:50	methanol/water/70% ethylamine (50:50:0.13)
AM2:1	acetonitrile–methanol (2 : 1) containing 0.1% ammonium formate (pH 6.4)
AMEa25:75:1	acetonitrile/methanol/triethylamine 25:75:1 (0.3% formic acid, pH 6.8)
AW5:95	acetonitrile/water (5:95) containing 0.05% formic acid
PMW5:1:4	isopropanol/methanol/water (5:1:4) supplemented with 0.2% formic acid, 0.028% ammonia, and 5 micro M phosphoric acid
PMW25:55:20	methanol (A), isopropanol (B), and water (containing 200 mM ammonium formate) (C), (A/B/C 55:25:20)

Mobile phase B

Abbreviation	Description
PAc0.1	isopropanol (0.1% acetic acid + 0.028% ammonia)
PFa0.2	isopropanol supplemented with 0.2% formic acid, 0.028% ammonia
PFa0.1	isopropanol containing 0.1% formic acid and 0.028% ammonia
PEa0.13	2-propanol/70% ethylamine (100:0.13)
MW2:1	methanol–H2O (2 : 1) containing 0.1% ammonium formate (pH 6.4)
PMW40:60:?	methanol (A), isopropanol (B), and water (containing 200 mM ammonium formate) (C), (A/B 60:40)
AW90:10	acetonitrile/H2O (90:10) containing 0.05% formic acid

Gradient program

Abbreviation	Description
IE	isocratic elution A:B 1:1 v/v
90/10:60/40:45/55	holding solvent (A/B:90/10) for 5 min linearly converting to solvent (A/B:60/40) for 35min linearly converting solvent (A/B:45/55) for 50 min
100/0:70/30:0/100	100% A for 10 min, increased 30%B at 60 min, 100%B for 15 min
95/5:10/90	95%/5% (A/B) to 10%/90% (A/B)
100/0:50/50:100/0	100:0 (0–5 min), 50:50(5–25 min), 50:50 (25–59 min), 100:0 (59–60 min), and 100:0(60–75 min)
100/0:95/5:40/60	100% A for 0–10 min linear gradient from 95%A/5% B to 40% A/60% B for 10–110 min

Mass Spectrometry

Ionization

Abbreviation	Description
ESI	electrospray ionization
FTICR	Fourier transform ion cyclotron resonance

MS/MS conditions

Abbreviation	Description
MRM	multiple reaction monitoring
NLS	neutral loss scan
PIS	precursor ion scan

Mode

Abbreviation	Description
Pos	positive
Neg	negative

System

Abbreviation	Description	Vendor
APEX	APEX III, FTICR mass spectrometer	Brucker Daltonics
LCQ	LCQ, ion trap mass spectrometer	Thermo Fisher Scientific
LTQ	LTQ, ion trap mass spectrometer	Thermo Fisher Scientific
LTQ-Orbi	LTQ-Orbitrap, mass spectrometer	Thermo Fisher Scientific
QTRAP	4000QTRAP, quadrupole-linear ion trap hybrid mass spectrometer	Applied Biosystems
Q-TOF II	Q-TOF II, quadrapole/time of flight hybrid mass spectrometer	Micromass
Q-TOF mi	Q-TOF micro, quadrapole/time of flight hybrid mass spectrometer	Micromass
Quatro	Quattro II, triple-stage quadrupole mass spectrometer	Micromass