Taguchi:Abbreviations
Extraction Methods
| Abbreviation | Description |
|---|---|
| B&D | Bligh and Dyer method |
| Me | Methanol |
| CM1:1 | methanol(2ml), chloroform(2ml), 1 N HCl(2ml), 2 M NaCl(0.15ml) |
| CM2:1 | chloroform-methanol (2:1; v/v) |
| CMW1:2:0.8 | chloroform-methanol-water (1:2:0.8; v/v/v) |
| CuSO4 | ??? |
| TX-114 | ??? |
| SPE | Solid Phase extraction |
| SiGel | chloroform, acetone, chloroform/methanol (7:3) |
| SiTLC | chloroform/methanol/water (65:25:4) |
| WCM | water chloroform methanol |
| WHM | water hexane methanol |
| AMW18:11:1 | acetonitrile/methanol/water 18:11:1 (0.1% ammonium formate, pH 7.8) |
| CM200:100:3 | chloroform chloroform/methanol(1:1) chloroform/methanol/28% aqueous ammonia/acetic acid (200:100:3:0.9) chloroform/methanol/HCl (6:6:1) |
| CMW30:60:8 | chloroform-methanol-water (30:60:8; v/v/v) chloroform-methanol-0.8 M sodium acetate (30:60:8; v/v/v) |
| GSH | 20 mM reduced glutathiglutathione, 30 mM Tris, and 150 mM NaCl |
Gradient Programs
Mobile phase A
| Abbreviation | Description |
|---|---|
| AMW19:19:2 | acetonitrile/methanol/water:19/19/2 (0.1% acetic acid + 0.028% ammonia) |
| AMW6:7:2 | acetonitrile/methanol/water:6/7/2 (0.1% ammonium formate pH 7.0) |
| AMW18:11:1 | acetonitrile/methanol/water 18:11:1 (0.1% ammonium formate, pH 7.8) |
| AMW2:2:1 | acetoniacetonitrile:methanol:water 2:2:1 v/v/v containing 0.1% formic acid and 0.028% ammonia |
| MW1000:1 | methanol-28% aqueous ammonium-water (1000 : 1 : 1, pH 11) |
| MW50:50 | methanol/water/70% ethylamine (50:50:0.13) |
| AM2:1 | acetonitrile–methanol (2 : 1) containing 0.1% ammonium formate (pH 6.4) |
| AMEa25:75:1 | acetonitrile/methanol/triethylamine 25:75:1 (0.3% formic acid, pH 6.8) |
| AW5:95 | acetonitrile/water (5:95) containing 0.05% formic acid |
| PMW5:1:4 | isopropanol/methanol/water (5:1:4) supplemented with 0.2% formic acid, 0.028% ammonia, and 5 micro M phosphoric acid |
| PMW25:55:20 | methanol (A), isopropanol (B), and water (containing 200 mM ammonium formate) (C), (A/B/C 55:25:20) |
Mobile phase B
| Abbreviation | Description |
|---|---|
| PAc0.1 | isopropanol (0.1% acetic acid + 0.028% ammonia) |
| PFa0.2 | isopropanol supplemented with 0.2% formic acid, 0.028% ammonia |
| PFa0.1 | isopropanol containing 0.1% formic acid and 0.028% ammonia |
| PEa0.13 | 2-propanol/70% ethylamine (100:0.13) |
| MW2:1 | methanol–H2O (2 : 1) containing 0.1% ammonium formate (pH 6.4) |
| PMW40:60:? | methanol (A), isopropanol (B), and water (containing 200 mM ammonium formate) (C), (A/B 60:40) |
| AW90:10 | acetonitrile/H2O (90:10) containing 0.05% formic acid |
Gradient program
| Abbreviation | Description |
|---|---|
| IE | isocratic elution A:B 1:1 v/v |
| 90/10:60/40:45/55 | holding solvent (A/B:90/10) for 5 min linearly converting to solvent (A/B:60/40) for 35min linearly converting solvent (A/B:45/55) for 50 min |
| 100/0:70/30:0/100 | 100% A for 10 min, increased 30%B at 60 min, 100%B for 15 min |
| 95/5:10/90 | 95%/5% (A/B) to 10%/90% (A/B) |
| 100/0:50/50:100/0 | 100:0 (0–5 min), 50:50(5–25 min), 50:50 (25–59 min), 100:0 (59–60 min), and 100:0(60–75 min) |
| 100/0:95/5:40/60 | 100% A for 0–10 min linear gradient from 95%A/5% B to 40% A/60% B for 10–110 min |
