Acidic glycolipids (gangliosides, sulphatides)

Lipids were extracted from mouse brain with chloroform–methanol (2:1, v/v) and then with chloroform–methanol-water (1:2:0.8, v/v/v).
The extraction was dried under a gentle stream of nitrogen and re-dissolved with 7.5 ml chloroform-methanol-water(30:60:8; v/v/v).
Samples were applied to a DEAE-Sephadex A-25 column (6 x 45 mm, acetate form) (Amersham Biosciences AB, Sweden).
Neutral lipids were eluted with 15 ml chloroform-methanol-water (30:60:8; v/v/v).
Acidic glycolipid mixtures were eluted with 7.5 ml chloroform-methanol-0.8 M sodium acetate (30:60:8; v/v/v).

クロロホルム:メタノール混合 (2:1, v/v), クロロホルム:メタノール:水混合 (1:2:0.8, v/v/v) で抽出
ゆるやかな窒素ガスで乾燥、7.5 ml クロロホルム:メタノール:水混合 (30:60:8; v/v/v) に再度溶解
DEAE-Sephadex A-25 カラム (6 x 45 mm, acetate form) (Amersham Biosciences AB, Sweden) にセット
中性脂質を 15 ml クロロホルム:メタノール:水混合 (30:60:8; v/v/v) で洗浄
酸性の糖脂質混合物を、 7.5 ml クロロホルム:メタノール:0.8M 酢酸ナトリウム混合 (30:60:8; v/v/v) で溶出

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