Acidic glycolipids (gangliosides, sulphatides)

Lipids were extracted from mouse brain (500 mg) with 9 ml chloroform–methanol (2:1, v/v) and then with 7.6 ml chloroform–methanol-water (1:2:0.8, v/v/v).
The extraction was dried under a gentle stream of nitrogen and re-dissolved with 7.5 ml chloroform-methanol-water(30:60:8; v/v/v).
Samples were applied to a DEAE-Sephadex A-25 column (6 x 45 mm, acetate form) (Amersham Biosciences AB, Sweden).
Neutral lipids were eluted with 15 ml chloroform-methanol-water (30:60:8; v/v/v).
Acidic glycolipid mixtures were eluted with 7.5 ml chloroform-methanol-0.8 M sodium acetate (30:60:8; v/v/v).

マウス脳　(500 mg)　からクロロホルム:メタノール混液 (2:1, v/v) 9 ml, クロロホルム:メタノール:水混液 (1:2:0.8, v/v/v) 7.6 ml で抽出
窒素ガス下で乾固、クロロホルム:メタノール:水混液 (30:60:8; v/v/v) 7.5 ml に再度溶解
DEAE-Sephadex A-25 カラム (6 x 45 mm, acetate form) (Amersham Biosciences AB, Sweden) にセット
中性脂質をクロロホルム:メタノール:水混合 (30:60:8; v/v/v) 15 ml で溶出
酸性の糖脂質混合物を、クロロホルム:メタノール:0.8M 酢酸ナトリウム混液 (30:60:8; v/v/v) 7.5 ml で溶出

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