Taguchi:Abbreviations: Difference between revisions

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=Methods=
{{Taguchi/Header}}
==Extraction==
{| class="wikitable sortable"
|-
! Abbreviation || Description
{{#repeat:Taguchi/Table/Row2|1|
&&{{ListPage|method|B&D}} && Bligh and Dyer method
&&{{ListPage|method|SPE}} && Solid-phase extraction method
&&{{ListPage|method|TLC}} && Thin layer chromatography
}}
|}


==Extraction Methods==
===Solvent===
{| class="wikitable sortable"
{| class="wikitable sortable"
|-
|-
! Abbreviation || Description
! Abbreviation || Description
{{#repeat:Taguchi/Table/Row2|1|
{{#repeat:Taguchi/Table/Row2|1|
&& B&D && Bligh and Dyer method &&
&&Ace && acetone
&& Me  && Methanol &&
&&AceA && acetic acid
&& CM1:1 && methanol(2ml), chloroform(2ml), 1 N HCl(2ml), 2 M NaCl(0.15ml) &&
&&ACN && acetonitrile
&& CM2:1 && chloroform-methanol (2:1; v/v) &&
&&Amm && ammonia
&& CMW1:2:0.8 && chloroform-methanol-water (1:2:0.8; v/v/v) &&
&&AmFo && ammonium formate
&& CuSO4 && ???
&&But && butanol
&& TX-114 && ???
&&Chl && chloroform
&& SPE && Solid Phase extraction
&&ForA && formic acid
&&Hex && hexane
&&IsoP && isopropanol
&&Met && methanol
&&MFo && methyl formate
&&NaAc && sodium acetate
&&PhoA && phosphoric acid
&&TriE && triethylamine
&&W && water
}}
|}
 
==Purification==
{| class="wikitable sortable"
|-
! Abbreviation || Description || Vendor
{{#repeat:Taguchi/Table/Row3|1|
&& C18 SP && C18 Sep-Pak && Waters
&& DEAEc && DEAE cellulose && Wako Pure Chemicals
&& DEAEsA25 && EAE sephadex A-25 && Amersham Biosciences
&& GS4B && glutathione Sepharose 4B && Amersham Biosciences
&& SiG && Silica gel &&
&& OHLB && Oasis HLB && Waters
}}
|}
 
==Liquid Chromatography==
===Phase===
{| class="wikitable sortable"
|-
! Abbreviation || Description
{{#repeat:Taguchi/Table/Row2|1|
&& Nor && normal
&& Rev && reverse
}}
|}
 
===Program===
{| class="wikitable sortable"
|-
! Abbreviation || Description
{{#repeat:Taguchi/Table/Row2|1|
&& Gra && gradient
&& Iso && isocratic
&& Lin && linear
&& Ste && stepwise
}}
|}
===Column===
{| class="wikitable sortable"
|-
! Abbreviation || Description || Vendor
{{#repeat:Taguchi/Table/Row3|1|
&& ACU18  && ACQUITY UPLC BEH C18 && Waters
&& APC18  && Acclaim PepMap300 C18 && LC PACKINGS
&& DevC30  && Deverosil C30 && Nomura Chemicals
&& DevSi60 && Deverosil Si60 && Nomura Chemicals
&& HiQ sil && HiQ sil  && KYA TECH
&& Si60    && Si60 && Nomura Chemicals
&& UC18    && Unison C18 nano LC column && Imtakt
&& WXBC18  && Waters X-Bridge C18 && Waters
}}
|}
 
===System===
{| class="wikitable sortable"
|-
! Abbreviation || Description || Vendor
{{#repeat:Taguchi/Table/Row3|1|
&& {{ListPage|system|AU}} && ACQUITY Ultra Performance LC system && Waters
&& {{ListPage|system|HP1100}} && HP 1100 HPLC system && Hewlett-Packard
&& {{ListPage|system|LC10AD}} && LC-10AD vp micro HPLC system && Shimadzu
&& {{ListPage|system|P305}} && pump model 305 && Gilson
&& {{ListPage|system|UM}} && Ultimate HPLC system && LC Packings
&& {{ListPage|system|UM3000}} && UltiMate 3000 nano/cap/micro LC system && Dionex
}}
|}
 
<!---------
===Program===
{| class="wikitable sortable"
|-
! Abbreviation || Description
{{#repeat:Taguchi/Table/Row2|1|
&& Gra && gradient
&& Iso && isocratic
&& Lin && linear
&& Ste && stepwise
}}
|}
 
==Parameters==
{| class="wikitable sortable"
|-
! Abbreviation || Description
{{#repeat:Taguchi/Table/Row2|1|
&&Me  && Methanol
&&CM1:1 && methanol(2ml), chloroform(2ml), 1 N HCl(2ml), 2 M NaCl(0.15ml)
&&CM2:1 && chloroform-methanol (2:1; v/v)
&&CMW1:2:0.8 && chloroform-methanol-water (1:2:0.8; v/v/v)
&&CuSO4 && ???
&&TX-114 && ???
&&SiGel&& chloroform, acetone, chloroform/methanol (7:3)
&&SiTLC && chloroform/methanol/water  (65:25:4)
&&WCM && water chloroform methanol
&&WHM && water hexane methanol
&&AMW18:11:1&& acetonitrile/methanol/water 18:11:1 (0.1% ammonium formate, pH 7.8)
&&CM200:100:3&& chloroform chloroform/methanol(1:1) chloroform/methanol/28% aqueous ammonia/acetic acid (200:100:3:0.9) chloroform/methanol/HCl (6:6:1)
&&CMW30:60:8&& chloroform-methanol-water (30:60:8; v/v/v) chloroform-methanol-0.8 M sodium acetate (30:60:8; v/v/v)
&&GSH&& 20 mM reduced glutathiglutathione, 30 mM Tris, and 150 mM NaCl
}}
|}
 
==Gradient Programs==
===Mobile phase A===
{| class="wikitable sortable"
|-
! Abbreviation || Description
{{#repeat:Taguchi/Table/Row2|1|
&&AMW19:19:2 && acetonitrile/methanol/water:19/19/2 (0.1% acetic acid + 0.028% ammonia)
&&AMW6:7:2 &&  acetonitrile/methanol/water:6/7/2 (0.1% ammonium formate pH 7.0)
&&AMW18:11:1 && acetonitrile/methanol/water 18:11:1 (0.1% ammonium formate, pH 7.8)
&&AMW2:2:1 && acetoniacetonitrile:methanol:water 2:2:1 v/v/v containing 0.1% formic acid and 0.028% ammonia
&&MW1000:1 &&  methanol-28% aqueous ammonium-water (1000 : 1 : 1, pH 11)
&&MW50:50 &&  methanol/water/70% ethylamine (50:50:0.13)
&&AM2:1 &&      acetonitrile–methanol (2 : 1) containing 0.1% ammonium formate (pH 6.4)
&&AMEa25:75:1 && acetonitrile/methanol/triethylamine 25:75:1 (0.3% formic acid, pH 6.8)
&&AW5:95 &&    acetonitrile/water (5:95) containing 0.05% formic acid
&&PMW5:1:4 &&  isopropanol/methanol/water (5:1:4) supplemented with 0.2% formic acid, 0.028% ammonia, and 5 micro M phosphoric acid
&&PMW25:55:20 && methanol (A), isopropanol (B), and water (containing 200 mM ammonium formate) (C), (A/B/C 55:25:20)
}}
|}
 
===Mobile phase B===
{| class="wikitable sortable"
|-
! Abbreviation || Description
{{#repeat:Taguchi/Table/Row2|1|
&&PAc0.1 && isopropanol (0.1% acetic acid + 0.028% ammonia)
&&PFa0.2 && isopropanol supplemented with 0.2% formic acid, 0.028% ammonia
&&PFa0.1 && isopropanol containing 0.1% formic acid and 0.028% ammonia
&&PEa0.13 && 2-propanol/70% ethylamine (100:0.13)
&&MW2:1 && methanol–H2O (2 : 1) containing 0.1% ammonium formate (pH 6.4)
&&PMW40:60:? && methanol (A), isopropanol (B), and water (containing 200 mM ammonium formate) (C), (A/B 60:40)
&&AW90:10 && acetonitrile/H2O (90:10) containing 0.05% formic acid
}}
|}
 
===Gradient program===
{| class="wikitable sortable"
|-
! Abbreviation || Description
{{#repeat:Taguchi/Table/Row2|1|
&&IE && isocratic elution A:B 1:1 v/v
&&90/10:60/40:45/55 && holding solvent (A/B:90/10) for 5 min linearly converting to solvent (A/B:60/40) for 35min linearly converting solvent (A/B:45/55) for 50 min
&&100/0:70/30:0/100 && 100% A for 10 min, increased 30%B at 60 min, 100%B for 15 min
&&95/5:10/90 && 95%/5% (A/B) to 10%/90% (A/B)
&&100/0:50/50:100/0 && 100:0 (0–5 min), 50:50(5–25 min), 50:50 (25–59 min), 100:0 (59–60 min), and 100:0(60–75 min)
&&100/0:95/5:40/60 && 100% A for 0–10 min linear gradient from 95%A/5% B to 40% A/60% B for 10–110 min
}}
|}
-------->
==Mass Spectrometry==
===Ionization===
{| class="wikitable sortable"
|-
! Abbreviation || Description
{{#repeat:Taguchi/Table/Row2|1|
&& ESI && electrospray ionization
&& FTICR && Fourier transform ion cyclotron resonance
}}
|}
 
===MS/MS conditions===
{| class="wikitable sortable"
|-
! Abbreviation || Description
{{#repeat:Taguchi/Table/Row2|1|
&& MRM && multiple reaction monitoring
&& NLS && neutral loss scan
&& PIS && precursor ion scan
}}
|}
 
===Mode===
{| class="wikitable sortable"
|-
! Abbreviation || Description
{{#repeat:Taguchi/Table/Row2|1|
&& Pos && positive
&& Neg && negative
}}
|}
 
===System===
{| class="wikitable sortable"
|-
! Abbreviation || Description || Vendor
{{#repeat:Taguchi/Table/Row3|1|
&& {{ListPage|system|APEX}} && APEX III, FTICR mass spectrometer && Brucker Daltonics
&& {{ListPage|system|LCQ}}&& LCQ, ion trap mass spectrometer && Thermo Fisher Scientific
&& {{ListPage|system|LTQ}} && LTQ, ion trap mass spectrometer && Thermo Fisher Scientific
&& {{ListPage|system|LTQ-Orbi}} && LTQ-Orbitrap, mass spectrometer && Thermo Fisher Scientific
&& {{ListPage|system|QTRAP}} && 4000QTRAP, quadrupole-linear ion trap hybrid mass spectrometer && Applied Biosystems
&& {{ListPage|system|Q-TOF II}} && Q-TOF II, quadrapole/time of flight hybrid mass spectrometer && Micromass
&& {{ListPage|system|Q-TOF mi}} && Q-TOF micro, quadrapole/time of flight hybrid mass spectrometer && Micromass
&& {{ListPage|system|Quatro}} && Quattro II, triple-stage quadrupole mass spectrometer && Micromass
}}
}}
|}
|}

Latest revision as of 08:10, 24 August 2010

Top Lipid Class Method Symbols

Extraction

Abbreviation Description
Bligh and Dyer method
Solid-phase extraction method
Thin layer chromatography

Solvent

Abbreviation Description
Ace acetone
AceA acetic acid
ACN acetonitrile
Amm ammonia
AmFo ammonium formate
But butanol
Chl chloroform
ForA formic acid
Hex hexane
IsoP isopropanol
Met methanol
MFo methyl formate
NaAc sodium acetate
PhoA phosphoric acid
TriE triethylamine
W water

Purification

Abbreviation Description Vendor
C18 SP C18 Sep-Pak Waters
DEAEc DEAE cellulose Wako Pure Chemicals
DEAEsA25 EAE sephadex A-25 Amersham Biosciences
GS4B glutathione Sepharose 4B Amersham Biosciences
SiG Silica gel
OHLB Oasis HLB Waters

Liquid Chromatography

Phase

Abbreviation Description
Nor normal
Rev reverse

Program

Abbreviation Description
Gra gradient
Iso isocratic
Lin linear
Ste stepwise

Column

Abbreviation Description Vendor
ACU18 ACQUITY UPLC BEH C18 Waters
APC18 Acclaim PepMap300 C18 LC PACKINGS
DevC30 Deverosil C30 Nomura Chemicals
DevSi60 Deverosil Si60 Nomura Chemicals
HiQ sil HiQ sil KYA TECH
Si60 Si60 Nomura Chemicals
UC18 Unison C18 nano LC column Imtakt
WXBC18 Waters X-Bridge C18 Waters

System

Abbreviation Description Vendor
ACQUITY Ultra Performance LC system Waters
HP 1100 HPLC system Hewlett-Packard
LC-10AD vp micro HPLC system Shimadzu
pump model 305 Gilson
Ultimate HPLC system LC Packings
UltiMate 3000 nano/cap/micro LC system Dionex

Mass Spectrometry

Ionization

Abbreviation Description
ESI electrospray ionization
FTICR Fourier transform ion cyclotron resonance

MS/MS conditions

Abbreviation Description
MRM multiple reaction monitoring
NLS neutral loss scan
PIS precursor ion scan

Mode

Abbreviation Description
Pos positive
Neg negative

System

Abbreviation Description Vendor
APEX III, FTICR mass spectrometer Brucker Daltonics
LCQ, ion trap mass spectrometer Thermo Fisher Scientific
LTQ, ion trap mass spectrometer Thermo Fisher Scientific
LTQ-Orbitrap, mass spectrometer Thermo Fisher Scientific
4000QTRAP, quadrupole-linear ion trap hybrid mass spectrometer Applied Biosystems
Q-TOF II, quadrapole/time of flight hybrid mass spectrometer Micromass
Q-TOF micro, quadrapole/time of flight hybrid mass spectrometer Micromass
Quattro II, triple-stage quadrupole mass spectrometer Micromass
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