Taguchi:Summary

メタボロミクス、特にリピドミクスにおける分析手法について

This page contains information of original papers that describe lipid analysis and identification at Taguchi Laboratory, Graduate School of Medicine, The University of Tokyo. The database introduces useful methodologies in lipidomics for each lipid class such as acylglycerol, phospholipid, and glycolipid. Papers indexed here are classified for each experimental step: extraction, purification, liquid chromatography, mass spectrometry, and identification. The papers are also linked to PubMed database.

このページは、東京大学医学系研究科田口研究室()における脂質の分析から同定までが記載されている論文を掲載しています。脂質の網羅的・包括的解析に有用な分析手法をそれぞれの脂質(たとえばアシルグリセロール、リン脂質、糖脂質など)に分けて、抽出、精製、液体クロマトグラフィー、マススペクトロメトリー、脂質同定するまでの過程を簡単明瞭に示しました。詳細な知識を得たいユーザーのために論文からPubMedの抄録のページへリンクを張ってあります。

Contents

Paper Summary

Total 16 original papers

paper ID authors title lipids MS LC
Taguchi:OP101
(PMID 17449863)

 
 
 
 
 
 
 

Changes in molecular species profiles of glycosylphosphatidylinositol anchor precursors in early stages of biosynthesis

 

 
 

 

Taguchi:OP104
(PMID 18206977)

 
 
 

Development of a reverse-phase liquid chromatography electrospray ionization mass spectrometry method for lipidomics, improving detection of phosphatidic acid and phosphatidylserine

 
 
 
 

 

 

Taguchi:OP110
(PMID 18703820)

 
 
 

Targeted analysis of ganglioside and sulfatide molecular species by LC/ESI-MS/MS with theoretically expanded multiple reaction monitoring

 
 
 
 
 
 
 
 
 
 
 

 

 

Taguchi:OP113
(PMID 18956880)

 
 

Reversed-Phase LC/MS Method for Polyphosphoinositide Analyses: Changes in Molecular Species Levels during Epidermal Growth Factor Activation in A431 Cells

 
 
 

 

 

Taguchi:OP114
(PMID 18964370)

 
 
 
 

Analysis of oxidized phosphatidylcholines as markers for oxidative stress, using multiple reaction monitoring with theoretically expanded data sets with reversed-phase liquid chromatography/tandem mass spectrometry

 

 

 
 

Taguchi:OP121
(PMID 19481987)

 
 
 
 

Global analysis of triacylglycerols including oxidized molecular species by reverse-phase high resolution LC/ESI-QTOF MS/MS

 
 

 

 

Taguchi:OP129
(PMID 19880374)

 
 
 
 

Separation and quantification of sn-1 and sn-2 fatty acid positional isomers in phosphatidylcholine by RPLC-ESIMS/MS

 
 

 

 

Taguchi:OP131
(PMID 20069690)

 
 
 
 
 

Liquid chromatography/mass spectrometry analysis revealing preferential occurrence of non-arachidonate-containing phosphatidylinositol bisphosphate species in nuclei and changes in their levels during cell cycle

 

 

 

Taguchi:OP134
(PMID 20452604)

 
 

Precise and global identification of phospholipid molecular species by an Orbitrap mass spectrometer and automated search engine Lipid Search

 
 
 
 
 
 

 

 

Taguchi:OP76
(PMID 10972995)

 
 
 
 

Two-dimensional analysis of phospholipids by capillary liquid chromatography/electrospray ionization mass spectrometry

 
 
 
 
 

 

 

Taguchi:OP87
(PMID 15384179)

 
 
 
 
 
 
 

High-resolution analysis by nano-electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry for the identification of molecular species of phospholipids and their oxidized metabolites

 
 
 
 

 
 

 

Taguchi:OP88
(PMID 15565732)

 
 
 
 
 
 

Rapid and selective identification of molecular species in phosphatidylcholine and sphingomyelin by conditional neutral loss scanning and MS3

 
 

 
 

 
 

Taguchi:OP90
(PMID )

 
 
 
 

Specific detection of lysophosphatidic acids in serum extracts by tandem mass spectrometry

 

 

Taguchi:OP91
(PMID 15700236)

 
 
 
 
 

A shotgun tandem mass spectrometric analysis of phospholipids with normal-phase and/or reverse-phase liquid chromatography/electrospray ionization mass spectrometry

 
 
 
 
 

 
 

 
 

Taguchi:OP93
(PMID 15990370)

 
 
 
 
 
 
 

Focused lipidomics by tandem mass spectrometry

 
 
 
 
 

 

 

Taguchi:OP94
(PMID 16407401)

 
 
 
 
 
 

PGAP2 Is Essential for Correct Processing and Stable Expression of GPI-anchored Proteins