Overview
Lipid contents exhibit high dynamic range. Detection of scarce elements is difficult as it is due to ion suppressions; different extraction methods are necessary depending on purposes.
- Sample preparation ... extraction depending on purposes
- Analytical strategy ... selection of platforms and methods
- Computational analysis ... qualitative/quantitative and multivariate analysis
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脂質は量的に多い成分と少ない成分のダイナミックレンジが大きいため、主要成分と混合したままだと微量成分の検出はイオンサプレッションなどにより難しくなる。つまり、目的に応じたサンプル調製が重要である。
- 前処理 ... 目的に応じたサンプル調製
- 分析 ... 装置と測定方法の選択
- 数理解析 ... 定性と定量ならびに多変量解析
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Amount & Species
abundant few
scarce many
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Sample Preparation
- Homogenize cells or organs with methanol
- Butanol extraction
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- 細胞・臓器をメタノールでホモジナイズ
- ブタノール抽出法
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Analytical Strategy
| Strategy |
Method |
Type |
Instrument
|
| Global (detection of all lipids)
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LC/MS
Data dependent scan (LC/MS/MS, LC/MS3)
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Hybrid type MS
- quadrupole-time-of-flight (Q-TOF)
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Hybrid type MS
- ion-trap (IT)-TOF or Fourier-Transform Ion Cyclotron Resonance (FT-ICR)
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- LTQ-Orbitrap (Theremo-Fisher Scientific)
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| Focused (focusing on partial structures)
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Precursor ion scan
Neutral loss scan
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Triple-quadrupole MS
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- 4000 Q-TRAP or 5500 Q-TRAP (Applied Biosystems)
- TSQ Quantum or Vantage (Thermo- Fisher Scientific)
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| Targeted (targeting by molecular and fragment ions)
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Multiple reaction monitoring (MRM),
Selected reaction monitoring(SRM)
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Computational Analysis
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